Instructions for Use - Glyco Kit MB-LAC AIA - Bruker

Figure 1 exemplarily depictured possible experimental strategies to facilitate the identification of
glyco-proteins, to enable the detection of N-glycosylation sites, to support the structural
characterization of N-glycosylated peptides and to perform glyco-profiling studies in the clinical
context.
General Information
Glycosylation is the most common post-translational modification of proteins, playing a key role in
protein folding, stability, solubility, activity and molecular recognition. Altered glycosylation is known
to act as an indicator or effector in pathologic mechanisms with potential impact on diagnosis and
therapy of human diseases. Immunity to certain infectious diseases, prion diseases and cancer
can all be associated with abnormal glycosylation patterns. As an example, increased branching
and sialylation of N-linked glycans on cancer cells are potential tumor markers in molecular
diagnostics.
Bruker Daltonics’ CLINPROT magnetic glyco-beads, specifically functionalized with lectins or
boronic acids that bind to various structural motifs, facilitate the selective isolation of different
glycoproteins out of complex biological sources (Fig. 1). Bruker Daltonics has developed workflows
combining the specific pre-fractionation employing glyco-specific magnetic particles, e.g. ConA,
WGA, LCA or boronic acids coated beads, and LC-MALDI analysis to identify glycoproteins, to
locate N-glycosylation sites and to get structural information about N-glycosylated peptides.
MB-LAC
Con A
MB-LAC
LCA
MB-LAC
WGA
MB-LAC
AIA
MB-CovAC
boronic
High-mannose type Hybrid type Complex type
Glycorotein
Glycoprotein
Glycoprotein
Man
GlcNAc
Gal
Fuc
NeuAc
GalNAc
Glycoprotein
Glycoprotein
Glycoprotein
O-linked glycans
C-linked glycans
N-linked glycans
Fig. 2: Binding motifs of Bruker Daltonics’ CLINPROT TM magnetic glyco-beads. The different beads
represent overlapping but individual binding profiles.
Successful applications and reproducibility of Bruker Daltonics’ CLINPROT TM magnetic glycobeads
using model systems or complex samples like serum, plasma or urine were demonstrated in
various studies 1,2,3 . Further, long term stability and storage conditions were extensively
investigated to assure the high quality of Bruker Daltonics’ CLINPROT TM magnetic glycobeads
4 .The application of glyco-beads in the context of glyco-profiling and biomarker discovery
was closely described by Drake et al. 5
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