Instructions for Use - Glyco Kit MB-LAC AIA - Bruker

Intended Use
The glyco kit MB-LAC-AIA (Magnetic Bead-Lectin Affinity Chromatography with Artocarpus
Integrifolia Agglutinin = Jacalin) is developed for the capturing of preferentially O-glycosylated
proteins derived from biological samples based on lectin affinity prior to MALDI-TOF mass
spectrometry analysis by means of e.g. Bruker´s microflex ® , autoflex ® or ultraflex ® MALDI-TOF.
This kit is for research use only. It is not for use in diagnostic procedures.
Product description
The MB-LAC AIA kit is designed for enrichment and purification of preferentially O-glycosylated
proteins prior to MALDI-TOF MS analysis. The MB-LAC AIA kit is based on super-paramagnetic
microparticles functionalized with Artocarpus Integrifolia Agglutinin.
Artocarpus Integrifolia Agglutinin (AIA) is a lectin which specifically binds the structure galactosyl
(-1-3) N-acetylgalactosamine and -D-galactose. Jacalin captures galactosyl (-1-3) N-
acetylgalactosamine even in a mono- or di-sialylated form 6 .
CH 2 OH
OH CH 2 OH
O
OH
OH
OH
O
O
OH
HN
O
Fig. 3: Structure of galactosyl-(ß-1,3)-N-acetylgalactosamine which is the main recognition motif of jacalin.
Due to the binding requirements AIA preferentially binds O-glycans. This lectin has been used to
exclusively purify human IgA without enriching any other human immunoglobulin class 7 .
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