Instructions for Use - Glyco Kit MB-LAC AIA - Bruker
Instructions for Use - Glyco Kit MB-LAC AIA - Bruker
Instructions for Use - Glyco Kit MB-LAC AIA - Bruker
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
General remarks <strong>for</strong> sample handling and MALDI measurements<br />
MALDI-TOF mass spectrometry is a very sensitive analysis method requiring special operation<br />
procedures. There<strong>for</strong>e, we recommend considering the following aspects be<strong>for</strong>e starting an<br />
analysis:<br />
The quality of the starting material will have an enormous impact on the outcome of the<br />
experiments. Especially, collection and storage conditions have to be controlled carefully.<br />
When working with clinical samples, like serum or urine, please refer to the recent<br />
publications of Baumann et al. 8 and Fiedler et al. 9 <strong>for</strong> standardized sample handling<br />
procedures.<br />
To avoid contaminations all solvents used should be of HPLC grade or better<br />
The plastic materials used <strong>for</strong> sample purification and matrix preparation should be<br />
explicitly recommended <strong>for</strong> mass spectrometric measurements. Many common plastics<br />
may cause polymer contamination of the sample. This will interfere with the subsequent<br />
matrix preparation/crystallization and produce polymer signals during spectra acquisition.<br />
Sample purification and MALDI target preparation should be per<strong>for</strong>med under clean<br />
conditions. Dust and air pollution will decrease the spectra quality.<br />
For optimal sample/matrix crystallization on the MALDI target the following environmental<br />
conditions are recommended:<br />
Operating temperature: 5 - 40°C (41 - 104°F)<br />
Operating humidity: 25 – 65 % at 22°C<br />
Atmospheric pressure: 75 - 105 kPa<br />
For high resolution measurements and low signal to noise values a cleaning of the ion<br />
source at regular intervals is absolutely mandatory. This procedure can be per<strong>for</strong>med with<br />
the <strong>Bruker</strong> Daltonics’ source shower target (see ordering in<strong>for</strong>mation). For a complete<br />
cleaning of the ion source please ask <strong>Bruker</strong> Daltonics’ service team<br />
(maldi.support@bdal.de).<br />
Page 6 of 19