Instructions for Use - Glyco Kit MB-LAC AIA - Bruker
Instructions for Use - Glyco Kit MB-LAC AIA - Bruker
Instructions for Use - Glyco Kit MB-LAC AIA - Bruker
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Analysis of deglycosylated peptides using PAC targets<br />
For the analysis of deglycosylated peptides (e.g. after PNGase F treatment) the use of PAC (Prespotted<br />
AnchorChip) targets prespotted with α-Cyano-4-hydroxycinnamic acid (HCCA) is<br />
recommended (#231968 or #227463).<br />
Sample preparation onto pre-spotted disposable AnchorChip targets<br />
1. Deposit 0.3 – 3 µl sample soluton onto<br />
the matrix spot. The acetonitrile<br />
concentration of the sample must not<br />
exceed 40 %<br />
Spot 0.3 – 3 µl sample<br />
2. Incubate the sample <strong>for</strong> 3 min onto the<br />
spot, but do not let the droplets dry<br />
3 min incubation<br />
3. Remove the residual solvent Remove droplet<br />
4. Add 7 – 10 µl of washing buffer (10mM<br />
Ammonium phosphate, monobasic, in<br />
0.1 % TFA)<br />
Wash with 7 – 10 µl<br />
5. Incubate <strong>for</strong> a few second and<br />
subsequently remove the wash buffer<br />
Remove wash buffer<br />
after a few seconds<br />
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