Instructions for Use - Glyco Kit MB-LAC AIA - Bruker
Instructions for Use - Glyco Kit MB-LAC AIA - Bruker
Instructions for Use - Glyco Kit MB-LAC AIA - Bruker
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Example<br />
Isolation of Asialofetuin from a protein mixture<br />
Sample:<br />
Protein mixture consisting of Protein Standard II (1 µl, #207234) and<br />
Asioalofetuin (AF, 1 µg)<br />
Beads:<br />
<strong>MB</strong>-<strong>LAC</strong> <strong>AIA</strong><br />
Target: MTP AnchorChip 600/384<br />
Matrix:<br />
2,5-DHAP<br />
MALDI mode: Linear<br />
Asiaolofetuin (43.2 kDa) was successfully isolated by <strong>MB</strong>-<strong>LAC</strong> <strong>AIA</strong> beads from a protein mixture<br />
consisting of BSA (66.4 kDa), Protein A (44.5 kDa) Trypsinogen (23.9 kDa) and Asialofetuin (43.2<br />
kDa). Only Asialofetuin was detected in the eluate and no residual Asiaolfetuin was detected in the<br />
supernatant.<br />
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