Soil Microbial Ecology - Soil Molecular Ecology Laboratory
Soil Microbial Ecology - Soil Molecular Ecology Laboratory
Soil Microbial Ecology - Soil Molecular Ecology Laboratory
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E X E R C I S E<br />
3<br />
SOIL MICROBIAL ENUMERATION:<br />
1. DILUTION PLATE METHOD<br />
OBJECTIVE<br />
To analyze frequency and density and to compare similarity of microbial community for<br />
soil comparative studies<br />
INTRODUCTION<br />
<strong>Soil</strong>s generally contain an enormous and extremely diverse population of<br />
microorganisms. Prokaryotes are the most abundant of these organisms. They consist of<br />
only a single cell that lacks a distinct nuclear membrane and has a cell wall of a unique<br />
composition. Prokaryotes are divided into two domains: Archaea (which include<br />
methanogens and extremophiles; e.g. extreme halophiles, and extreme thermophiles); and<br />
Bacteria, which include the vast majority of prokaryotes.
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