Soil Microbial Ecology - Soil Molecular Ecology Laboratory

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LABORATORY SCHEDULE
08/29 Field Trip Sampling Forest and Garden Soils
09/12 Exercise 1 Mycorrhizal quantification experiment ...............................8 – 13
09/19 Exercise 2 Initiate soil decomposition experiment..............................14 - 16
09/26 Exercise 3 Dilution plating..................................................................17 - 23
00/03 Exercise 4 Microscopy ........................................................................24 - 37
Observe bacteria ..........................................................28 - 32
Start Riddell mounts ....................................................33
10/10 Exercise 5 Direct counts ......................................................................38 - 39
Observe fungi...............................................................33 - 37
10/17 Exercise 6 Initiate rhizobium experiment............................................40 - 43
10/24 Exercise 7 Initiate algae experiment....................................................44 - 49
Phosphatase assay ........................................................47 - 49
10/31 Exercise 8 Initiate respiration experiment...........................................50 - 56
11/07 Collect WK1 respiration data
11/14 Collect WK2 respiration data
11/21 Exercise 9 DNA analyses ...................................................................57 - 65
11/28 Collect mycorrhizal data
12/05 Exercise 10 Soil rhizosphere ................................................................66 - 67
12/07 End Classes
LABORATORY REPORTS
Report Grade Date Title Comment
1 15% 10/11 Enumeration of
bacteria & fungi
2 15% 11/21 Quantification of
microbial activity
Critical discussion of methods
employed Microbial observations
Soil Respiration/Biomass and
Phosphatase activity