Soil Microbial Ecology - Soil Molecular Ecology Laboratory
Soil Microbial Ecology - Soil Molecular Ecology Laboratory
Soil Microbial Ecology - Soil Molecular Ecology Laboratory
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E X E R C I S E<br />
9<br />
SOIL METABOLIC ASSESMENT<br />
3. DNA EXTRACTION AND QUANTIFICATION<br />
OBJECTIVE<br />
Use MO BIO protocol to extract DNA of two contrasting soils<br />
INTRODUCTION<br />
Use kit MO BIO Catalog # 12800-100 for isolating DNA from 0.25 - 1gm soil samples.<br />
Please wear gloves and avoid all skin contact with protocol materials. In case of accident contact,<br />
wash thoroughly with water and do not ingest. See Material Safety Data Sheets for emergency<br />
procedures in case of accidental ingestion or contact. Reagents labeled flammable should be kept<br />
away from open flames and sparks.<br />
MATERIALS:<br />
Micro centrifuge (10,000 x g)<br />
Pipette (volumes required 50 µl - 500 µl), and<br />
Vortex<br />
Kit Contents: 2 ml Bead Solution tubes (contains 550µl solution) 100<br />
Description<br />
Amt.<br />
Solution S1<br />
6.6 ml<br />
IRS solution<br />
22 ml<br />
Solution S2<br />
27.5 ml<br />
Solution S3<br />
143 ml<br />
Solution S4<br />
30 ml<br />
Solution S5<br />
6 ml<br />
Spin filters units in 2 ml tubes 100<br />
Collection tubes (2 ml) 300
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