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Soil Microbial Ecology - Soil Molecular Ecology Laboratory

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Page 31<br />

GRAM-NEGATIVE AEROBIC RODS AND COCCI<br />

Pseudomonadaceae<br />

Pseudomonas - straight or curved rods, motile by polar flagella, chemoorganotrophs,<br />

most are strict aerobes (a few species can denitrify), abundant in rhizosphere.<br />

Xanthomonas - straight rods, motile by polar flagellum, growth on agar yellow,<br />

chemoorganotrophs, strict aerobes, mostly plant pathogens.<br />

Azotobacteraceae<br />

Azotobacter - large ovoid to coccoid cells, marked pleomorphism, form thick-walled<br />

cysts and capsular slime, motile with peritrichous flagella or nonmotile, gram-neg. or<br />

variable, fix atmospheric nitrogen.<br />

Beijerinckia - straight to pear-shaped with rounded ends, up to 6 um with occasional<br />

branching, cysts and capsules in some species, produces copious slime in culture,<br />

fixes atmospheric nitrogen, acid tolerant.<br />

Rhizobiaceae<br />

Rhizobium - rods but pleomorphic under adverse conditions, motile by 2 to 6 peritrichous<br />

flagella, nonsporing, copious extracellular slime in culture, chemoorganotrophs,<br />

aerobic to microaerophilic, able to invade root hairs of leguminous plants.<br />

Agrobacterium - rods, motile by 1 to 4 peritrichous flagella, nonsporing, slime<br />

production, chemoorganotrophs, aerobic, most initiate plant hypertrophies.<br />

Uncertain affiliation<br />

Alcaligenes - rods to cocci, motile by up to 4 peritrichous flagella, chemoorganotrophs,<br />

strict aerobes. Saprophytes in animals and soil.<br />

Gram-Negative Facultative Anaerobic Rods<br />

Flavobacterium - coccobacilli to slender rods, motile with peritrichous flagella or<br />

nonmotile, pigmented in culture, chemoorganotrophs, fastidious as to requirement.<br />

Gram-Negative Cocci and Coccobacilli<br />

Acinetobacter - short rods to cocci, large irregular cells and filaments in culture, no<br />

spores or flagella, chemoorganotrophic, strict aerobes, resistant to penicillin.<br />

Gram-Positive Cocci<br />

Micrococci - spherical, non-motile, chemoorganotrophs, aerobic.<br />

Staphylococcus - spherical, chemoorganotrophs, metabolism respiratory or fermentative,<br />

produce extracellular enzymes and toxins, facultative anaerobes.<br />

Streptococcus - spherical to ovoid, chemoorganotrophs, metabolism fermentative,<br />

facultative anaerobes.

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