Soil Microbial Ecology - Soil Molecular Ecology Laboratory
Soil Microbial Ecology - Soil Molecular Ecology Laboratory
Soil Microbial Ecology - Soil Molecular Ecology Laboratory
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Page 56<br />
QUESTIONS:<br />
1. Why is the barium chloride added to the absorption vessel prior to titration?<br />
2. What is the source of the carbon dioxide recovered in the nonamended soils?<br />
How did the addition of glucose and cellulose affect microbial respiration -<br />
why?<br />
3. What factors influence the reliability of these methods as an index of<br />
microbial activity?<br />
4. How would you expect CO 2 evolution from a soil amended with groundsoybean<br />
plants to compare to that of a soil amended with straw? Support your<br />
answer!<br />
REFERENCES:<br />
Bartha, R. and Pramer, D. 1965. Features of a flask and method for measuring the<br />
persistence and biological effects of pesticides in soil. <strong>Soil</strong> Science 100:68-70.<br />
Hendricks, C.W. and Pascoe, N. 1988. <strong>Soil</strong> microbial biomass estimates using 2450<br />
MHz microwave irradiation. Plant and <strong>Soil</strong> 110:39-47.<br />
Horwath, W.R. and E.A. Paul. 1994. <strong>Microbial</strong> biomass. p. 753-773. In R.W. Weaver and<br />
et al. (ed.) Methods of soil analysis, Part 2, Microbiological and biochemical properties --<br />
SSSA book series, no. 5. <strong>Soil</strong> Science Society of America, Madison, WI .
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