Soil Microbial Ecology - Soil Molecular Ecology Laboratory
Soil Microbial Ecology - Soil Molecular Ecology Laboratory
Soil Microbial Ecology - Soil Molecular Ecology Laboratory
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Page 54<br />
BIOMASS (DEMO ONLY):<br />
Treatments are: 2 soil samples (Agriculture and Forest) and 2 microwave irradiation (+ or<br />
-)<br />
METHOD<br />
Weigh 50 g dry mass of well-mixed soil (soil should contain about 10% moisture) and<br />
place in Erlenmeyer flask of a Biometer Vessel (see fig. below).<br />
One vessel from each soil is microwaved for 30 seconds (high power, 700 W, 2450 MHz<br />
microwave). Note, this is sufficient to kill approximately 90% of the cell. After<br />
microwaving, close vessel with rubber stopper.<br />
A similar vessel is assembled with non-microwaved soil.<br />
Charge side tubes with exactly 10 ml of 1.0 N Na0H and seal.<br />
After 10 days, titrate the NaOH as above to determine, the CO 2 evolved.<br />
Calculate the mg C biomass by the formula:<br />
(HCl used in control - HCl used in microwaved soil) / 0.5 1 = mg C biomass<br />
(Assuming 50% conversion of biomass to C in 10 days)
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