Methodologies Basmati Rice & Bushmeat

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Variety SOP FOOD STANDARDS AGENCY STANDARD OPERATING PROCEDURE (SOP) 3 Version 1.0, October, 2007 STANDARD OPERATING PROCEDURE FOR THE DETECTION OF NON-PERMITTED RICE VARIETIES BY INDEL-PCR ANALYSIS USING THE AGILENT 2100 BIONALYZER • Available for use in-house • Available as a service from Tepnel and other genetic analysis providers
Quantitative test • Original test uses area under curve • Preferred DNA quantification of mixtures is via Real-Time PCR • Agilent method restricted to analysis of electrophoresis peaks • Decision to be made regarding baseline / threshold for measurement • Has implications for quantitative analysis
Page 1 and 2: Methodologies Basmati Rice & Bushme
Page 3 and 4: Intensity Existing authentication m
Page 5 and 6: Indel markers 3’ 5’ 3’ 5’ S
Page 7 and 8: Size Example output 1 2 3 4 5 6 7 8
Page 9: Band profiles Basmati Group 1 B1 B5
Page 13 and 14: Bushmeat Identification • Surveys
Page 15 and 16: Methodology Restriction Fragment Le
Page 17 and 18: Example results • Fragment catego
Page 19: Acknowledgements Katherine Steele,

Methodologies Basmati Rice & Bushmeat

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