Methodologies Basmati Rice & Bushmeat

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Species identification • Species identification of truly unknown samples requires DNA sequencing • Sequencing is not possible at most PA labs • Species detection tests easier to perform • Test exist for many common species exist • Aim to develop a test covering a range of illegally imported African wild species: • Chimpanzee, Gorilla, Mandrill • Duiker, Bushbuck, Porcupine • Cane rat, Grass cutter, Pouched rat • Bush pig, Zebu, African sheep
Methodology Restriction Fragment Length Polymorphism (RFLP) • Amplify common fragment by PCR (mitochondrial cytochrome b gene) • Digest with two restriction enzymes • Generate fragment profile to match against references HinfI MseI Species A Species B Species C
Page 1 and 2: Methodologies Basmati Rice & Bushme
Page 3 and 4: Intensity Existing authentication m
Page 5 and 6: Indel markers 3’ 5’ 3’ 5’ S
Page 7 and 8: Size Example output 1 2 3 4 5 6 7 8
Page 9 and 10: Band profiles Basmati Group 1 B1 B5
Page 11 and 12: Quantitative test • Original test
Page 13: Bushmeat Identification • Surveys
Page 17 and 18: Example results • Fragment catego
Page 19: Acknowledgements Katherine Steele,

Methodologies Basmati Rice & Bushmeat

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