BioDocAnalyze (BDA) digital - Biometra

Gel Documentation System
BioDocAnalyze (BDA) digital
Order Numbers
034-000, 034-050, 034-312, 034-313
Instructions for Installation
and
Image Acquisition
Software Version 2.64.11.20
9-990-017/english
Version 01_11
Please read the operating instructions carefully
before using the system!
Biometra GmbH
D-37079 Göttingen Service
Rudolf-Wissell-Straße 30 Rudolf-Wissell-Str. 14-16
D-37079 Göttingen D-37079 Göttingen
Tel.: +49 551 50686-0 Tel.: +49 551 50881-10/12
Fax: +49 551 50686-66 Fax: +49 551 50881-11
e-mail: Info@biometra.com Service@biometra.com
internet: http://www.biometra.de

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All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means
without the prior written permission of Biometra biomedizinische Analytik GmbH nor be otherwise circulated electonically or in print.
In no event Biometra biomedizinische Analytik GmbH shall be liable for indirect, incidential or consequential damages resulting from the
use of the information supplied by this manual. This manual was written with due care and Biometra biomedizinische Analytik GmbH shall
not be liable for any fault or omission.
March 2011.
Tradenames of software or hardware cited herein, esp. those of the Microsoft Corp., are registered, patented or protected by other legal
means.
BDAdigital Manual, Vers. 01_11

Contents
BDAdigital Manual, Vers. 01_11
3
Contents
1 INSTALLATION OF BDA DIGITAL ...........................................................................................................4
1.1 COMPONENTS ...........................................................................................................................................4
1.2 ASSEMBLY ................................................................................................................................................4
1.2.1 Personal computer and monitor........................................................................................................4
1.2.2 Copy protection (Dongle, Hardlock)................................................................................................4
1.2.3 Attaching of the lens adapter to the camera .....................................................................................5
1.2.4 Installation of camera with anti-theft mounting to the darkhood BDR 1 .........................................5
1.2.5 Mounting of camera at BDA Box ....................................................................................................6
1.2.6 Bandpass Filter.................................................................................................................................8
1.2.7 Filter wheel labels „590 nm EtBr“ and „520 nm SYBR ® Green“....................................................8
1.2.8 Darkhood, transilluminator and converter plate...............................................................................8
1.2.9 Thermal printer.................................................................................................................................8
1.3 INSTALLATION OF THE SOFTWARE............................................................................................................9
1.3.1 Overview..........................................................................................................................................9
1.3.2 Installation of the Camera Canon PowerShot G10/G9/A640/A620/G6/G5/G3/G2 .........................9
1.3.3 Screen Settings...............................................................................................................................11
1.3.4 Installation of the BioDocAnalyze Program ..................................................................................11
1.3.5 Installation of Thermal Printer Mitsubishi P93DW .......................................................................12
2 START OF THE PROGRAM.....................................................................................................................13
2.1 CHOICE OF MAIN WINDOWS.....................................................................................................................13
3 BDA DIGITAL IMAGE CAPTURE............................................................................................................14
3.1 THE IMAGE CAPTURE..............................................................................................................................15
3.2 FUNCTIONS AND CAPTURE PARAMETERS................................................................................................15
3.2.1 Profiles with Capture Parameters...................................................................................................15
3.2.2 The Card „Settings“ .......................................................................................................................16
3.2.3 The Card „Advanced“ ....................................................................................................................18
3.2.4 Camera Settings for Typical Gels ..................................................................................................19
3.2.5 Further Functions for Image Capture .............................................................................................20
3.2.6 The Gel Picture Window................................................................................................................22
5 SERVICE ......................................................................................................................................................23
6 EQUIPMENT DECONTAMINATION CERTIFICATE ............................................................................24
7 DISPOSAL ...................................................................................................................................................26

4
Installation
1 Installation of BDAdigital
concerning
034-000 BDAdigital core set
034-050 BDAdigital compact
034-312 BDAdigital System 20
034-313 BDAdigital System 30
1.1 Components
The system comes with:
- BDA software (CD-ROM) and manual
- Dongle (copy protection key, hardlock)
- Digital camera, e.g. Canon PowerShot incl. Canon standard accessories
- Lens adapter
- Bandpass filter BP595 for EtBr stained gels
Additionally with BDAdigital compact:
- Darkhood BDR1
Additionally with BDAdigital System 20 and BDAdigital System 30:
- darkhood BDA Box 2 incl. camera mounting
- Transilluminator UVstar 20 resp. UVstar 30
- UV converter plate
- Thermal printer incl. USB cable
- TFT monitor
- Personal computer, Englisch OS, installed
1.2 Assembly
1.2.1 Personal computer and monitor
Attach the cable of the monitor to the VGA port of the graphic card of the PC.
Connect also mouse and keyboard to the computer. Connect monitor and PC via cable with the appropriate
power supply system.
1.2.2 Copy protection (Dongle, Hardlock)
The BioDocAnalyze software is protected by a copy protection key (dongle, hardlock). The dongle is the software
licence.
The dongle is supplied as key for the USB port of the computer.
This dongle is plugged to the USB port of the computer.
After installation the dongle is automatically detected and flashes in red.
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BDAdigital Manual, Vers. 01_11
5
Installation
1.2.3 Attaching of the lens adapter to the camera
Please screw off the ring around the lens counter-clockwise by pressing the button next to the lens.
The bayonet nut connector gives the ring free after about a quarter of turn.
After this please put the lens adapter over the lens and screw it clockwise while pressing the button until it is
locked in place.
Applying the black small darkhood BDR1 of BDAdigital compact or BDA Box 1:
For documentation of Ethidiumbromide stained gels please screw the red bandpass filter BP595 to the lens
adapter.
For documentation of colored samples, e.g. silver or Coomassie Blue stained gels you don´t need a filter.
Applying the camera A640 we recommend to put the included rubber ring to the lower edge of the lens adapter.
This supports a light-tight sealing to the hood.
1.2.4 Installation of camera with anti-theft mounting to the darkhood BDR 1
(Models Canon PowerShot G10, G9, A640, A620, G6, G5 or G3)
Screw the lock block to the tripod thread of the camera with the included screw and the allen wrench. The lock
block must be fixed to the camera in the way that the two noses of the block are equally positioned against the
camera (see markers below):
lens adapter
band pass filter
Place the camera at the darkhood and attach the lock bolt.
The bolt and the safety pins must be inserted into the corresponding holes.
Turn the key clockwise at 180 ° and take it off.

6
Installation
The camera is finally fixed with the knurled screw.
1.2.5 Mounting of camera at BDA Box
With darkhood type BDA Box please mount the camera as follows:
1)
Ready to use
Knurled head screws
Hexagon socket
Camera bracket
Adapter bloc
In case of changing
the UV filter:
After removal of the knurled screw the camera
can be lifted and easily turned to the side. Now
it is possible to change the UV filter.
It is not possible to take off the camera!
It remains locked!
Digital camera (first unscrew
lens ring)
Lens adapter with O-Ring
Light trap
BDAdigital Manual, Vers. 01_11

2)
3)
Side view
For an optimal camera position the camera must
be adjusted in height that way that the mounted
O-Ring leaves 5,6 mm free at the lower part of the
lens adapter (see adjoining picture).
Mounting of the anti-theft device, back view
Special screw driver
Special screws
Security bracket
BDAdigital Manual, Vers. 01_11
O ring
Front view
7
Installation
Lichtfalle, oberer Teil
Light trap, upper part

8
Installation
1.2.6 Bandpass Filter
Standard delivery is a bandpass filter with transmission maximum of 590 nm for the typically used fluorescent
stain ethidium bromide.
With darkhood BDA Box 2 or BDA Box 3 please insert the UV filter into the filter wheel: Open the darkhood
sliding door and open the UV protection shield up to a horizontal position. Slowly pull out the filter wheel with
the knob inside the darkhood, below the filter wheel. The filter wheel is slided out and the filter can be inserted
into the wheel. Please choose the wheel position which is opposite to the corresponding filter label (see 1.2.7).
Note:
Filters with slightly higher filter holder shall be inserted in borehole Nr. 3 of the filter wheel. Then Nr. 1
is seen at filter wheel front.
1.2.7 Filter wheel labels „590 nm EtBr“ and „520 nm SYBR ® Green“
Delivery of a BDA Box includes two small filter wheel labels.
These labels can be applied to darkhoods with filter wheel (BDA Box 2, BDA Box 3) at the front panel of the
filter wheel. Please note that the visible front position number corresponds to the opposite filter wheel position.
The standard delivery of BDA systems includes the red „590 nm/ethidium bromide filter“, the green „520
nm/SYBR Green filter“ can be bought as optional component.
1.2.8 Darkhood, transilluminator and converter plate
Please refer to the separate operating instructions of the darkhood and the transilluminator.
The converter plate for application on top of the UV transilluminator for documentation of samples with visible
colour stains is delivered ready to use.
1.2.9 Thermal printer
! To avoid any Windows problems with the USB ports it is recommended to connect USB devices to
the same USB port as used for installation of the devices.
Connect the thermal printer Mitsubishi P93DW under Windows XP/2000 via USB cable with the PC. Connect
the printer with the power supply system and put a roll of paper into the printer.
Windows XP and 2000 are automatically recognizing the printer which is connected via USB cable as a new
instrument. Therefore the printer should only be switched on just before it shall be installed. To avoid installation
errors we recommend to install the thermal printer as last component of the system.
BDAdigital Manual, Vers. 01_11

1.3 Installation of the Software
BDAdigital Manual, Vers. 01_11
9
Installation
BDAdigital has been developed for the operating systems Windows XP professional and Windows 2000
professional.
The installation is only necessary in case you bought the sets 034-000 and 034-050 without a computer
from Biometra. If BioDocAnalyze comes with a Biometra computer, all the software is completely installed
and the system is ready to use after unpacking and installing the cables.
PC minimum requirements: Personal computer with Windows XP or Windows 2000, processor 1,8 GHz, graphic
card 1024 x 768, 512 MB RAM, 2 USB2.0 ports, CD-ROM drive.
Software installation: This will take, depending on the computer and how often the user did things like that before,
between 15 and 45 minutes. In case you have some problems during or after installation, please contact the
Biometra BioDocAnalyze customers service, phone +49(0)551-5068612, fax +49(0)551-5088111, e-mail
Herbert.Becker@whatman.com.
1.3.1 Overview
! For installation procedure please refer to following chapters with detailed information.
completed
1.3.1.1 Installation of camera (s. 1.3.2) .................................................................................... �
Installation of digital camera driver from Canon CD-ROM.
Afterwards connect camera to PC.
1.3.1.2 Screen settings (s. 1.3.3)............................................................................................... �
1.3.1.3 Installation of BDA software incl. dongle (s. 1.3.4) .................................................... �
from BDA CD-ROM
1.3.1.4 Installation of thermal printer (s. 1.3.5)........................................................................ �
from BDA CD-ROM
1.3.2 Installation of the Camera Canon PowerShot
G10/G9/A640/A620/G6/G5/G3/G2
!
The camera driver from the Canon Digital Camera Solution CD must be installed
before connecting the camera to the PC!
1.3.2.1 Installation of the Canon programs
Users of Windows XP professional and Windows 2000
must be logged in as system administrator!
1. Insert Canon Digital Camera Solution CD into PC drive.

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Installation
2. Choose „Installation of Digital Camera Software“.
3. Confirm request.
4. Confirm software licence agreement.
5. Confirm or choose the installation path.
6. Choose desired programs and start installing.
It depends on operating system and camera model whether or which USB driver for the camera has to be
installed.
- Windows XP/G10, G9, A640, A620, G6, G5: No USB driver must be installed.
- Windows XP/G3, G2: WIA driver for Canon cameras
- Windows 2000/G5, G3, G2: TWAIN driver for Canon cameras.
When the camera shall also be used separately from the BDA software for taking other pictures it is recommended
to install all Canon programs.
7. Reboot the computer.
1.3.2.2 Basic camera settings
The camera is not yet connected to the PC.
1. Switch the camera G2 resp. G3 with the mode switch to record or play, activate the menue and set „auto
power down“ to „off“ on card settings. Otherwise the camera will switch off automatically after some minutes
of inactivity which causes a program breakdown the next time BioDocAnalyze asks for the camera.
With camera G10, G9, A640, A620, G6 and G5 it is not necessary to set it manually because these cameras
automatically deactivate the auto power down function in their PC mode.
2. To have correct dates on your pictures, the time and date in the cameras menue, card settings, must be set.
3. Connect the camera with mains adapter with the power supply system.
4. In control panel, energy options the standby mode of the computer and the switch off of the harddisk
should be set to „never“ as after reactivation the connection to the camera may be lost. Switching off the
computer monitor after some minutes of inactivity or the screen saver will not disturb the camera function.
After this connect the camera to the computer by its USB cable.
! To avoid any Windows problems with the USB ports it is recommended to connect USB devices to
the same USB port as used for installation of the devices.
Differences in camera models:
G9, G10: Switch on the camera with main switch „on/off“.
A640: Please set the switch on the camera back to play „ �“ and switch on the camera with the main switch
“ON/OFF”.
The camera models G6, G5 and G3 will switch on automatically, if the camera´s loaded battery is installed or the
mains adaptor is connected. With camera model G2. the mode switch has to be set to play „ �“.
The camera automatically switches to the PC mode which is shown on the small upper camera display (not
displayed with A640).
! In case that the Canon program or Windows window for operating the camera is automatically
opened please close it.
BDAdigital Manual, Vers. 01_11

1.3.3 Screen Settings
BDAdigital Manual, Vers. 01_11
11
Installation
Set it with „Start, control panel, appearance and themes, change screen resolution“ (WinXP) resp. „Start, settings,
control panel, display, settings“ (Win 2000):
- Screen area: 1024 x 768 pixels
- Colors: True Color (32 bit)
Set „Start, control panel, appearance and themes“ (WinXP):
- font size to normal
Win2000:
The function „Use large icons“ on the index card „effects“ should not be activated.
1.3.4 Installation of the BioDocAnalyze Program
incl. the driver for the copy protection key (hardlock, dongle)
1. Insert the BioDocAnalyze installation CD into the PC drive.
2. Start „my computer“ or the Windows Explorer and choose the CD-ROM drive.
3. Start installation with double-click on the file „BDAdigital_setup.bat“ in the main directory.
Now the drivers for the dongle and the BioDocAnalyze software are installed.
4. Confirm the request for completing the installation.
Note:
Starting the BDAdigital image acquisition window for the first time you are asked to type in a 4-digit activation
code to get free access to this software part. You will find the requested code labeled to the copy
protection key (see also section 3).
********************************************************************
It is also possible to install the BioDocAnalyze software and the drivers of the dongle separately:
BioDocAnalyze software Start the file „setup.exe“ in the main directory of the BioDocAnalyze CD and confirm
all requests.
Dongle Start the file „hdd32.exe“ in the directory „HASP“ and confirm all requests.
********************************************************************
BioDocAnalyze Software Update
When the BioDocAnalyze software shall be installed to an existing system as software update only the Bio-
DocAnalyze software has to be installed newly.
A new installation of other drivers and programs is not necessary.
Installation:
1. Please uninstall the existing BioDocAnalyze software version by „Start, control panel, Software, BioDocAnalyze“
with the„delete“ button.
2. Now please install the BioDocAnalyze software from CD by double-click on the file „setup.exe“ in the
main directory of the BioDocAnalyze CD.
3. Confirm all requests.

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Installation
Restart your computer. Switch on the thermal printer before so you will be asked for the printer driver automatically.
1.3.5 Installation of Thermal Printer Mitsubishi P93DW
The default settings for printing in the configuration file biom1.ini of the BioDocAnalyze program are optimized
for the thermal printer Mitsubishi P93DW with thermal paper KP65HM or KP91HG-CE and for the thermal
printer Sony UP-D895 with Sony UPP-110HG paper. In case another printer or another paper are used the
printouts can be too dark. Then an individual adaptation of setting values (printcontrast, printbrightness, printgamma)
in the biom1.ini is recommended.
1.3.5.1 Installation under Windows 2000 and Windows XP
The drivers for Windows XP and Windows 2000 are in the directory MitsubishiP93DW.
The printer installation at the USB port runs widely automatically. Please confirm the different requests as recommended
from the system. In case that there are any open questions please refer to the Sony installation guide
on the BioDocAnalyze CD in directory MitsubishiP93DW.
A possibility to use the thermal paper more efficiently is to activate the paper save mode in the printer settings:
Choose „Start, settings, printer & fax“ and open the dialog box of the thermal printer P93D. Choose card „settings“
and set the paper save mode to „on“.
BDAdigital Manual, Vers. 01_11

2 Start of the Program
BDAdigital Manual, Vers. 01_11
13
Start of Program
The program is started from the desktop by a LEFT MOUSE BUTTON DOUBLECLICK on to the icon "BioDocAnalyze"
or alternatively the command sequence "Start, Programs,...,BioDocAnalyze".
The BioDocAnalyze-Logo is opened and after several seconds displaced by the full frame window "BioDoc-
Analyze" with a menu bar and a function bar.
2.1 Choice of main windows
The desired function is activated by a click on the icon in the function bar. You have
the choice between
� Image capture with CCD video camera (BDAvideo)
� Image capture with digital camera (BDAdigital)
� Image capture with digital CCD camera (BDA live)
� Gel analysis.
This manual only refers to the image capture with the digital camera.
The gel analysis is described in the separate manual „BioDocAnalyze“.

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Image Capture
3 BDAdigital Image Capture
The BDAdigital image capture software is started with a LEFT MOUSE BUTTON CLICK on the
icon „digital camera“.
! When starting the BDAdigital capture software for the
first time after installation you will be asked for a 4-digit
software code to activate the capture screen.
The code comes along with the dongle.
The window „Capture digital camera“ is opened and a loading bar indicates that the software just initializes the
digital camera.
Fig. 3-1 Window „Capture digital camera“
The line „Camera“ should show e.g. „PowerShot AG10“. In case that only „Model“ is shown the camera is not
correctly installed or not switched on. The camera needs to be on replay and then shows „PC“ on the camera
display (display is not existing at all camera models).
When the capture window is opened it starts with the camera settings of the last opened profile.
BDAdigital Manual, Vers. 01_11

3.1 The Image Capture
These are the typical steps for image capture:
1. Switch on the live preview: .
2. Place the electrophoresis gel on the transilluminator.
3. Switch on the light source of the darkhood.
4. Place the gel with the help of the live preview in the transilluminator center.
5. Choose the desired zoom area by window „Settings“:
6. Choose the appropriate settings profile:
7. Take a photo:
BDAdigital Manual, Vers. 01_11
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Image Capture
8. If necessary optimize the exposure time Tv and the lens aperture Av in window „Settings“ in set „Shooting
Mode“ „Manual“ and take another photo.
9. Save and print the image:
Please see details of the different functions in section 3.2.
3.2 Functions and Capture Parameters
The window „Capture digital camera“ is widely self-explaining and very similar to general known parameters in
photography. In spite of that the individual functions are explained in short in the following.
3.2.1 Profiles with Capture Parameters
Profile This includes some profiles with working parameters for the image acquisition of certain gel types.
A mouse click on the profile will set the parameters in the camera. The corresponding parameters are
shown in the cards Settings and Advanced. It is essential that the Shooting Mode is „manual“ otherwise
the settings shown in the card Settings will not really be the used parameters.
It is also possible to enter names for individual settings. After typing in the new name and setting the
parameters the new profile has to be saved .
Switch off of the „live image“ will accelerate the change of profiles.
Save This saves user-defined profiles under the active profile name.
Before saving you should check whether already a profile with the same name exists because it
would be overwritten.
Clear This deletes the active profile. The Biometra saved profiles can not be deleted.

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Image Capture
3.2.2 The Card „Settings“
Shooting Mode For choice of capture mode. Typical settings are „Manual“ or „Auto“.
- Manual Exposure time and lens aperture can be set in lines „Av“ and „Tv“. Even for the use of
profile parameters the mode should be „Manual“.
It should be kept in mind that the camera can not set the largest apertures (= small aperture
number) together with a large zoom factor. In these cases the camera automatically
chooses the largest possible lens aperture. After the picture is taken the aperture display on
the card is actualized to the really used one.
- Auto The camera sets the capture parameters exposure time and lens aperture automatically.
Be aware that these settings are not displayed in the camera settings.
Be aware that the displayed and printed settings are not the true settings the cameras has
automatically chosen for the photo!
- Tv The camera automatically chooses the optimal lens aperture in relation to the manually
set exposure time. After the picture is taken the automatically used aperture is displayed in
the card Settings.
- Av The camera automatically chooses the optimal exposure time in relation to the manually
set lens aperture. After the picture is taken the automatically used exposure time is displayed
in the card Settings.
Lens aperture, Av For setting the lens aperture. A small aperture number stands for a wide open lens, a high
aperture number for a more closed lens. The depth of focus increases with rising aperture
number (= smaller lens aperture). Agarose gels, especially thicker gels, should be photographed
with high aperture number (= small aperture). Otherwise photos may not be focussed.
Exposure time, Tv For setting the exposure time. The time is to be set in dependance of sample/illumination
and lens aperture. For smaller apertures (= high aperture number, e.g. 8) exposure times of
up to 8 seconds can be necessary for a photo of fluorescent gels.
Resolution For choice of image resolution in pixels. The higher the resolution the bigger the file size.
High resolutions will cause longer data transfer times from camera to PC on computers
with slow processors and memory below 512 MB RAM. For an efficient operating it is
recommended to use a computer with at least 1 GB RAM and a 2.8 GHz processor.
In pixels PowerShot G10 PowerShot G9 PowerShot A640
High
Medium1
Medium2
Low
4416 x 3312
3456 x 2592
2592 x 1944
1600 x 1200
4000 x 3000
3264 x 2448
2592 x 1994
640 x 480
3648 x 2736
2816 x 2112
2272 x 1704
640 x 480
PowerShot
A620/G6
3072 x 2304
2592 x 1944
2048 x 1536
640 x 480
Zoom The optical zoom can be set with the slider or by typing in a value between 0 and 13 (G10,
G9), 0 and 8 (A640/A620), 0 and 12 (G6/G5/G3) resp. 1 to 10 (G2).
A higher zoom reduces the amount of light that reaches the camera sensors. This can cause
difficulties for the autofocus. When the autofocus does not succeed to take a focussed
picture although there is a clear object in the image center please
- set a longer exposure time (Shooting Mode Manual)
- set less zoom.
- Use the function “AF Lock” for fixation of the autofocus. (see 3.2.5)
- use the function „manual Focus“ (MF button on camera back). This manual focus is
not available for use with remote control by the computer. For use of the manual focus
please disconnect the USB cable of the camera from the PC and run the camera
BDAdigital Manual, Vers. 01_11

BDAdigital Manual, Vers. 01_11
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Image Capture
separately. Please set also the macro mode (flower symbol on camera back). The photos
are saved on the compact flash card.
Fig. 3-2 Card „Settings“

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Image Capture
3.2.3 The Card „Advanced“
Quality For choice of compression of picture data in JPG format. In general quality „normal“
will be sufficient. Quality „superfine“ with only low compression needs more saving capacity
and time for image analysis.
ISO For setting of the sensitivity. Value ISO 400 is recommended for low illumination and big
zoom, but in contrast to photos at ISO 50 a certain background noise appears.
In most cases it is advised to use ISO 50. Only for the acquisition of very faint signals at
high zoom factors it can be necessary to raise the sensitivity to ISO 400.
Flash For activation or deactivation of the flash. Applications with darkhoods should be done
with flash „off“. Flash „on“ or „auto“ can be used for photographs with stand in a dark
room, but reflections on the gel surface can not be excluded.
Exposure
Compensation
For setting an exposure correction. A fine-tuning of the optimal exposure can be done in
small steps.
This function is not available in shooting mode „Manual“ because lens aperture and exposure
time are specifically set in the manual mode.
White Balance For setting the white balance. Colors can be mirrored more true to original when the
illumination source is set. This factor is of only minor relevance for black&white photos.
Autofocus
Distance
For definition of distance where the autofocus will look for the sample. For image acquisition
with Biometra darkhoods „Close up“ has to be set. This setting will lead within the
limited distance of camera to gel to well-focussed pictures over the whole range of zoom.
Fig. 3-3 Card „Advanced“
BDAdigital Manual, Vers. 01_11

3.2.4 Camera Settings for Typical Gels
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19
Image Capture
The software provides camera settings for two typical gel types in Profiles. Starting with these settings it is very
easy and fast to optimize the camera settings for individual gels.
Gel type EtBr Stained Agarose Gel
on UV table
Coloured Polyacrylamide Gel
on UV table with yellow converter
plate, without UV filter on
camera lens,
e. g. silver or Coomassie Blue
stained Polyacrylamide gel
Profile „DNA/EtBr“ Profile „Converter“
Shooting Mode Manual Manual
Lens aperture Av 6.3 4.0
Exposure time Tv 4 s 1/125 s
Autofocus distance Close up Close up
ISO 50 50
Flash Off Off
Quality Normal Normal
Resolution Medium 1 Medium 1
Zoom e. g. 5 e. g. 5
Exposure compensation 0 0
White balance Auto Fluorescent

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Image Capture
3.2.5 Further Functions for Image Capture
AF Lock Function for fixation of the autofocus. This function can be used for
photos which cannot be focussed with the autofocus. This may happen
with gels which have no bands or have only very weak bands in the gel
center or when using a high zoom.
The principle of this function is that first a clear bright tool is placed in
the center and the autofocus is directed to that. After fixing the autofocus
in this position the tool is removed and the photo can be taken.
Please note:
When you are changing the zoom you have to set the autofocus again!
Procedure:
A. with fluorescent agarose gels:
a) Switch on white light from above (+ place a thin ruler on the gel),
activate the autofocus button (or take a photo),
activate the AF Lock function,
switch off the white light from above (and remove the ruler),
switch on the UV light,
take the photo.
b) place a fluorescent ruler on the gel,
activate the autofocus button (or take a photo),
activate the AF Lock function,
remove the ruler,
take the photo.
B. with coloured gels on converter plate:
Place a non-fluorescent ruler on the gel,
activate the autofocus button (or take a photo),
remove the ruler,
take the photo.
Delay With activated Delay function the camera updates the internal settings
with a 5 seconds delay after the last change of settings parameters in
the cards Setting and Advanced. The use of the delay function is recommended
for a faster operating if the user wants to change several
parameters before taking the next picture.
If it is intended only to change one parameter it is more practical to
deactivate the delay function. Then the camera will react directly after
the parameter on the card is changed.
Refresh It is possible to manually trigger the updating of internal camera
settings with the Refresh button. Then it is not necessary to wait the 5
seconds standard delay for automatic updating.
Live image on/off For switch on/off the live preview in window „Live Preview“. Due to
the high iteration frequency of the picture and for this reason short exposure
times it is possible that the live preview will not be as bright and
focussed as the real gel picture.
Autofocus For manual activation of the autofocus. This can be helpful in case
that the camera fails to focus automatically.
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21
Image Capture
Take picture Click on the button releases the camera. The picture is transferred from camera to PC and
is displayed in the gel window in the right of the Capture window.
The “additional” functions Rotate, Invert, Black&White Mode and Saturation are deactivated
after taking a photo or printing. These functions have to be activated again when it is
desired to have.
Rotate 180° For rotating the picture 180°, for example when the gel is positioned the opposite way
round on the illumination table or when the camera is fixed on the darkhood or stand with
the camera control panel ahead.
Invert For inverting of the gel picture. The use of this function is only senseful with
black&white pictures.
Black&White Mode Activation of this function results in a change of the colour picture to a grey-scale picture.
Saturation Activation of this function sets areas of the photo to blue colour if the signal reaches saturation.
The photo display automatically switches to black&white mode.
The pixels of the area to be quantitatively analyzed later must not be saturated, neither
true white or true black. That means that the area of interest must not be in blue colour.
This can be achieved by shorter exposure times or with smaller lens apertures (= larger
lens aperture number).
When using longer exposure times it is possible that the live preview is darker than the
photo. This will cause differences in the saturation display of preview and photo.
Copy Conditions Click on this button loads the settings parameters of the gel picture (BD1 format!)
which was loaded into the gel window. The new setting parameters can now be used for
taking a new picture or for saving as profile.
Analysis For saving of the picture in the Biometra-specific BD1 format, JPG or TIF format and the
following transfer to the BDA analysis software. After saving the gel picture will automatically
be opened as black&white picture in the analysis part for further gel analysis.
Exit For closing of the capture window.
Fig. 3-4 Functions for image capture

22
Image Capture
File open Via the folder icon already saved pictures in JPG, TIF or Biometra specific BD1 format
can be loaded to the capture window.
Save Via disc icon the active gel picture is saved in JPG, TIF or Biometra specific BD1 format
by typing in a name. Default is BD1 format which generates a black&white image
including image acquisition camera settings. BD1 is standard format in the gel analysis
part of the BioDocAnalyze software.
Print Via printer icon the active gel picture is printed on the current Windows standard printer
(DIN A4 printer or thermal printer).
Thermal printer
mode
For prints with a thermal printer (e.g. Sony UP-D890 or UP-D895) the function „Thermal
printer mode“ has to be activated.
Print parameter It can be chosen whether the gel picture is printed with or without the camera settings.
Fig. 3-5 Save, load, and print file
3.2.6 The Gel Picture Window
Fig. 3-6 Cards of the gel picture window
Image Display of the photo or loaded gel picture.
Shot Conditions Setting parameters of the gel photo or the loaded gel file are shown.
The complete parameters are only saved with photos in BD1 files.
Comments For addition of file comments. These comments are only saved in BD1-format files!
The comments are printed with the gel photo and can also be seen in the BioDocAnalyze
analysis window under „Analyze, Comments, General Comment“.
Please regard: It is only possible to print around 20 chars per line and 5 lines.
BDAdigital Manual, Vers. 01_11

4 Service
BDAdigital Manual, Vers. 01_11
23
Service
In case you have any problem with the device please contact our service division or your local Biometra
dealer.
Biometra GmbH
Service Department
Rudolf-Wissell-Straße 14-16, D-37079 Göttingen, Germany
Tel: +49 551 50686-10 or -12
Fax: +49 551 50881-11
e-mail: service@biometra.com
Please follow the return instructions when returning the device:
Return instructions
Please contact our service department for providing a return authorization number (RAN). This
number has to be applied clearly visible to the outer box.
Returns without the RAN will be not be accepted!
Return only faulty devices. For any technical problem that is not clearly identifiable as a device
failure, please contact the Service Department of Biometra.
Use the original packing or a similar robust packing when returning the material.
The darkhood must be shipped on a pallet. The transilluminator has to removed from the
hood and must be shipped separately! The drawer must be secured with some packing
material to avoid that the drawer is moving and touching the UV protection shield during
transport.
Mark the outer packing with “CAUTION! SENSITIVE ELECTRONIC INSTRUMENT!”
Please include a precise description of the problem and preferably one that also describes by which
procedure the failure occurs or how it is caused.
Important:
Clean every part of the device from residues such as chemical, radioactive and dangerous
biological contamination. Please confirm in writing (Equipment Decontamination
Certificate) before every return that the device is free of radioactive and dangerous biological
contamination. Contaminated devices sent to Biometra will be refused!
The sender will be liable for the repair order in case of damage resulting from an inadequate
decontamination of the device.
Please include a note with the following information:
a) Sender’s name and address
b) Contact person and phone number in case of inquiry.

24
Service
5 Equipment Decontamination Certificate
To enable us to comply with german law (i.e. §71 StrlSchV, §17 GefStoffV and §19 ChemG) and to
avoid exposure to hazardous materials during handling or repair, please complete this form, prior to
the equipment leaving your laboratory.
COMPANY / INSTITUTE ____________________________________________________________________
ADDRESS ________________________________________________________________________________
PHONE NO _______________________________ FAX NO _______________________________________
E-MAIL ___________________________________________________________________________________
EQUIPMENT Model Serial No
__________________ __________________
__________________ __________________
__________________ __________________
__________________ __________________
If on loan / evaluation Start Date: ________________ Finish Date ____________________
Hazardous materials used with this equipment
_________________________________________________________________________________
_________________________________________________________________________________
_________________________________________________________________________________
Has the equipment been cleaned and decontaminated? YES / NO (delete)
Method of cleaning / decontamination
_________________________________________________________________________________
_________________________________________________________________________________
_________________________________________________________________________________
NAME ________________________________ POSITION ___________________________
(HEAD OF DIV./ DEP./ INSTITUTE / COMPANY)
SIGNED ______________________________ DATE _______________________________
PLEASE RETURN THIS FORM TO BIOMETRA GMBH OR YOUR LOCAL BIOMETRA DISTRIBU-
TOR TOGETHER WITH THE EQUIPMENT.
PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING. INSTRUMENTS WITHOUT
THIS CERTIFICATE ATTACHED WILL BE RETURNED TO SENDER.
BDAdigital Manual, Vers. 01_11

General Information for Decontamination:
Please contact your responsible health & safety officer for details.
Use of radioactive substances:
Please contact your responsible person for details.
Use of genetically change organism or parts of those:
Please contact your responsible person for details.
BDAdigital Manual, Vers. 01_11
25
Service

26
Service
6 Disposal
Due to the application in a laboratory the system must be decontaminated before return as electronic
waste.
Hinweis
Note
Renseignement
für die Entsorgung von Elektroaltgeräten
for disposal of electric / electronical
waste
du traitement des déchets des appareils
électrique / électronique
Das Symbol der durchgestrichenen Abfalltonne bedeutet für unsere Kunden in Deutschland, daß dieses Produkt
von der Firma Biometra kostenlos zur Entsorgung zurückgenommen wird.
Zu diesem Zweck kontaktieren Sie bitte unsere Service-Abteilung!
Außerhalb Deutschlands wenden Sie sich bitte an den für Sie zuständigen Händler.
Dieses Symbol gilt nur in Staaten des EWR*.
*EWR = Europäischer Wirtschaftsraum, umfasst die EU-Mitgliedsstaaten sowie Norwegen, Island und Liechtenstein.
----------------------------------------------------------------------------------------------------------------------------------------------------------------------
This symbol (the crossed-out wheelie bin) means, that this product should be brought to the return or separate
systems available to end-users according to yours country regulations, when this product has reached the end of
its lifetime.
For details, please contact your local distributor!
This symbol applies only to the countries within the EEA*.
*EEA = European Economics Area, comprising all EU-members plus Norway, Iceland and Liechtenstein.
----------------------------------------------------------------------------------------------------------------------------------------------------------------------
Cet symbole (conteneur à déchets barré d´une croix) signifie que le produit, en fin de vie, doit être retourné à un
des systèmes de collecte mis à la disposition des utilisateurs finaux en conséquence des régulations par la loi de
votre pays.
Pour des information additionel nous Vous demandons de contacter votre distributeur!
Cet symbole s´applique uniquement aux pays de l´EEE*.
*EEE = Espace économique européen, qui regroupe les États membres de l´UE et la Norvège, Islande et le
Liechtenstein.
BDAdigital Manual, Vers. 01_11