BioDocAnalyze (BDA) digital - Biometra

20
Image Capture
3.2.5 Further Functions for Image Capture
AF Lock Function for fixation of the autofocus. This function can be used for
photos which cannot be focussed with the autofocus. This may happen
with gels which have no bands or have only very weak bands in the gel
center or when using a high zoom.
The principle of this function is that first a clear bright tool is placed in
the center and the autofocus is directed to that. After fixing the autofocus
in this position the tool is removed and the photo can be taken.
Please note:
When you are changing the zoom you have to set the autofocus again!
Procedure:
A. with fluorescent agarose gels:
a) Switch on white light from above (+ place a thin ruler on the gel),
activate the autofocus button (or take a photo),
activate the AF Lock function,
switch off the white light from above (and remove the ruler),
switch on the UV light,
take the photo.
b) place a fluorescent ruler on the gel,
activate the autofocus button (or take a photo),
activate the AF Lock function,
remove the ruler,
take the photo.
B. with coloured gels on converter plate:
Place a non-fluorescent ruler on the gel,
activate the autofocus button (or take a photo),
remove the ruler,
take the photo.
Delay With activated Delay function the camera updates the internal settings
with a 5 seconds delay after the last change of settings parameters in
the cards Setting and Advanced. The use of the delay function is recommended
for a faster operating if the user wants to change several
parameters before taking the next picture.
If it is intended only to change one parameter it is more practical to
deactivate the delay function. Then the camera will react directly after
the parameter on the card is changed.
Refresh It is possible to manually trigger the updating of internal camera
settings with the Refresh button. Then it is not necessary to wait the 5
seconds standard delay for automatic updating.
Live image on/off For switch on/off the live preview in window „Live Preview“. Due to
the high iteration frequency of the picture and for this reason short exposure
times it is possible that the live preview will not be as bright and
focussed as the real gel picture.
Autofocus For manual activation of the autofocus. This can be helpful in case
that the camera fails to focus automatically.
BDAdigital Manual, Vers. 01_11