BioDocAnalyze (BDA) digital - Biometra

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18 Image Capture 3.2.3 The Card „Advanced“ Quality For choice of compression of picture data in JPG format. In general quality „normal“ will be sufficient. Quality „superfine“ with only low compression needs more saving capacity and time for image analysis. ISO For setting of the sensitivity. Value ISO 400 is recommended for low illumination and big zoom, but in contrast to photos at ISO 50 a certain background noise appears. In most cases it is advised to use ISO 50. Only for the acquisition of very faint signals at high zoom factors it can be necessary to raise the sensitivity to ISO 400. Flash For activation or deactivation of the flash. Applications with darkhoods should be done with flash „off“. Flash „on“ or „auto“ can be used for photographs with stand in a dark room, but reflections on the gel surface can not be excluded. Exposure Compensation For setting an exposure correction. A fine-tuning of the optimal exposure can be done in small steps. This function is not available in shooting mode „Manual“ because lens aperture and exposure time are specifically set in the manual mode. White Balance For setting the white balance. Colors can be mirrored more true to original when the illumination source is set. This factor is of only minor relevance for black&white photos. Autofocus Distance For definition of distance where the autofocus will look for the sample. For image acquisition with Biometra darkhoods „Close up“ has to be set. This setting will lead within the limited distance of camera to gel to well-focussed pictures over the whole range of zoom. Fig. 3-3 Card „Advanced“ BDAdigital Manual, Vers. 01_11
3.2.4 Camera Settings for Typical Gels BDAdigital Manual, Vers. 01_11 19 Image Capture The software provides camera settings for two typical gel types in Profiles. Starting with these settings it is very easy and fast to optimize the camera settings for individual gels. Gel type EtBr Stained Agarose Gel on UV table Coloured Polyacrylamide Gel on UV table with yellow converter plate, without UV filter on camera lens, e. g. silver or Coomassie Blue stained Polyacrylamide gel Profile „DNA/EtBr“ Profile „Converter“ Shooting Mode Manual Manual Lens aperture Av 6.3 4.0 Exposure time Tv 4 s 1/125 s Autofocus distance Close up Close up ISO 50 50 Flash Off Off Quality Normal Normal Resolution Medium 1 Medium 1 Zoom e. g. 5 e. g. 5 Exposure compensation 0 0 White balance Auto Fluorescent
Page 1 and 2: Gel Documentation System BioDocAnal
Page 3 and 4: Contents BDAdigital Manual, Vers. 0
Page 5 and 6: BDAdigital Manual, Vers. 01_11 5 In
Page 7 and 8: 2) 3) Side view For an optimal came
Page 9 and 10: 1.3 Installation of the Software BD
Page 11 and 12: 1.3.3 Screen Settings BDAdigital Ma
Page 13 and 14: 2 Start of the Program BDAdigital M
Page 15 and 16: 3.1 The Image Capture These are the
Page 17: BDAdigital Manual, Vers. 01_11 17 I
Page 21 and 22: BDAdigital Manual, Vers. 01_11 21 I
Page 23 and 24: 4 Service BDAdigital Manual, Vers.
Page 25 and 26: General Information for Decontamina

BioDocAnalyze (BDA) digital - Biometra

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