IFCPAR AR (ENGLISH) for CD - CEFIPRA

CEFIPRA
Centre Franco-Indien pour la Promotion de la Recherche Avancée
Duration: Three years and nine months (April, 2008 to
December, 2011)
Objectives
This project aims to study various immune parameters in
different categories of human population resident in Eastern
(malaria endemic) and Western (urban malaria non-endemic)
regions of India, and determine the possible relationship of
immune and genetic markers with resistance/susceptibility to
malaria.
The objectives are:
a) To analyse the self-antibody-mediated immune responses
in these different groups of patients
b) To monitor thymic T cell output T cell receptor excision
circle (TREC) analysis
c) To perform genetic analysis of certain host-genes involved
in immunomodulation, for both the innate and adaptive
immunity (TLR2, TLR4, TLR9, IFN γ, TNF α,
IL-10, IL-4, and
TGF β)
Accomplishments
i) Setting up of cohorts of more than 300 individuals from
controls to P. falciparum (Pf) infected patients divided in
several groups: Mild (MM), Severe Non Cerebral (SNCM),
Multi Organs Dysfunction (MOD), Cerebral Malaria (CM)
and Cerebral malaria with multi organs dysfunction (CM-
MOD). Two controls groups with septicaemia and
encephalitis have been added to the study
ii) Definition of 26 plex cytokine profiles in severe and non
severe Pf malaria
iii) Analysis of repertoire of autoantibody produced against
brain, liver, lung and kidney- definition of marker linked to
malaria severity;
iv) Analysis of parasite antigen-specific humoral response -
Correlation of the spectrum of parasite specific antibody
response with severe and non severe Pf malaria in Orissa
v) Analysis of genetic factors using SNPs for TLR-4, TNF- α (-
238 and -308, both G to A; iNOS (-954 G to C, -1173 C to
T-1173 C to T); IL-10 (-819 C to T, -592 C to A; -1082 G
to A); TGF β1 (+10 T to C, +25 G to C); IFNγR1 (-56 T to
C) in Pf patients and in controls
Research papers published: One
Project 3703-2
ROLE OF IMMUNE AND GENETIC FACTORS IN THE
OUTCOME OF PLASMODIUM FALCIPARUM MALARIA
Research Activities 2010-11
Life and Health Sciences
Dr. Shobhona Sharma
Department of Biological Sciences
Tata Institute of Fundamental
Research
Mumbai
Dr. Sylviane Pied
Centre d’Immunologie et de Biologie
Parasitaire
Institut Pasteur de Lille, INSERM U 547
Lille
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