IFCPAR AR (ENGLISH) for CD - CEFIPRA

CEFIPRA
Centre Franco-Indien pour la Promotion de la Recherche Avancée
Project 4103-1
EPIGENETICS OF TRANSCRIPTION BY RNA POLYMERASE III
Duration: Three years (October 2009 to September, 2012)
Objectives
i) To investigate the link between local chromatin structure
and expression level of the genes transcribed by the yeast
RNA polymerase III at global scale
ii) To characterize epigenetic regulation of local chromatin
structure, under different conditions affecting pol III
transcription
iii) To explore the role of local chromatin structure and
transcription activity in determining the gene locations in
the nuclear space
Accomplishments
i) Both positioned and fuzzy Nucleosomes were found near
the pol III-transcribed genes
ii) Most of the genes show an arrangement found typically
near pol II-transcribed genes; TSS in an NFR bordered by
positioned nucleosomes
iii) These nucleosomes change positions under repressed
state, pre-dominantly at 3'-ends of the genes
iv) Expression of different genes shows different response to
nutrient starvation
v) Different pol III-transcribed genes were found in different
locations within the nucleus
vi) Nuclear location of different genes shows different
response to nutrient deprivation
Research papers published: Nil
Research Activities 2010-11
Life and Health Sciences
Dr. Purnima Bhargava
Transcription and Chromatin lab
Centre for Cellular and Molecular
Biology
Hyderabad
Dr. Olivier Gadal
Equipe Organisation et Dynamique
Nucleaire
LBME du CNRS
Université Paul-Sabatier
Toulouse
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