IFCPAR AR (ENGLISH) for CD - CEFIPRA

CEFIPRA
Centre Franco-Indien pour la Promotion de la Recherche Avancée
Duration: Three years (September, 2009 to August, 2012)
Objectives
i) To develop practical and versatile synthetic methods to
furnish a wide variety of linear, functional molecules to
form densely packed Self Assembled Monolayers (SAMs)
on different types of surfaces like gold or silica
ii) To affect a single-step, post-assembly attachment of
desired functional groups to suitable terminal functions of
SAMs using alkene metathesis and/or 'Click chemistry'
(alkyne-azide cycloaddition)
iii) To graft covalently bioactive molecules and
macromolecules on suitably functionalised SAMs and
determine the extent and nature of grafting with relevant
spectroscopic and microscopic techniques e. g. atomic
force microscopy (AFM)
iv) To characterise the solid substrates at each step by surface
analytical methods (XPS, IR, ellipsometry, SPR, QCM-D)
and NMR spectroscopy (for disperse media) en route to
such biofunctionalisation. The interfacial reactions will be
monitored by ITC (isothermal titration calorimetry)
v) To evaluate the biological and biosensing activities of the
anchored biomolecules on solid supports; two major
identified objectives pertain to prevention of biofilm
formation on surfaces and development of novel
nanobiosensors
Accomplishments
i) Surface functionalization with Fischer carbene complex by
click chemistry
ii) Covalent grafting of proteins and peptide nucleic acid onto
gold-coated glass and silicon substrates by Fischer carbene
aminolysis
iii) Interfacial antigen- antibody recognition studies
iv) Interfacial PNA-DNA hybridization studies
v) Bacteriocidal surface by chemisorption of lysozyme
Research papers published: Five
Papers presented in conferences: 3
Posters: 2
Project 4105-1
PRACTICAL METHODS FOR
SURFACE BIOFUNCTIONALIZATION
Research Activities 2010-11
Pure & Applied Chemistry
Prof. Amitabha Sarkar
Department of Organic Chemistry
Indian Association for the Cultivation
of Science
Kolkata
Dr Michèle Salmain
Laboratoire Charles Friedel
Ecole Nationale Supérieure de Chimie
de Paris
Paris
A) Surface with immobilized protein A
B) Surface with rabbit IgG attached with protein A, and
C) Surface with protein A , rabbit IgG and goat anti-rabbit IgG
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