Relevance of Sperm DNA Fragmentation - eshre

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DNA fragmentation - Tests• DIRECT– TUNEL (terminal deoxynucleotydil dil transferasemediateddeoxyuridin triphosphate- nick-end labelingassay ) (s&dSB)– ISNT (in situ-nick translation)– Comet Assay at neutral pH (sdSB) (single cell gelelectrophoresis• INDIRECT (need denaturation of DNA)– SCSA (sperm chromatin structure assay)– SCD (sperm chromatin dispersion test, HalospermAssay)– Comet Assay at acid or basic pH (sdSB)
TUNEL assayLabels single and double strand breaks, quantifies the incorporation of flourescent dUTP
Page 1 and 2: Relevance of Sperm DNAFragmentation
Page 3: 1. Sperm DNA peculiarityDuring sper
Page 6 and 7: In mature sperm the DNA is• 85% p
Page 8: 2. Etiology of DNA Damagethe etiolo
Page 11 and 12: ROS• Small levels are essential f
Page 13: 3. DNA Fragmentation TestsDFI: DNA
Page 17 and 18: SCD or Halo TestThe SCD is based on
Page 20 and 21: 4. Influence of lab procedures
Page 23: The potential detrimental effect of
Page 26 and 27: 90% of patients showed no strandbre
Page 28 and 29: abSwim-up for maximum 2 hoursEbner
Page 30 and 31: DFImotilityRaw semen 15.8 ± 7.8% 3
Page 32 and 33: Why do fast progressive sperms show
Page 34 and 35: 5. Is DFI testing relevant forferti
Page 36 and 37: Fertility potential, threshold leve
Page 38 and 39: • in the general population:1-4%
Page 43 and 44: 23 papers that investigated the inf
Page 46 and 47: CONCLUSIONSsperm DNA damage is asso
Page 48 and 49: When oocytes from infertile patient
Page 50 and 51: Conclusions II• Small but signifi
Page 53 and 54: (infertile patients!)Infertile Pati

Relevance of Sperm DNA Fragmentation - eshre

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