Relevance of Sperm DNA Fragmentation - eshre

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abSwim-up for maximum 2 hoursEbner et al, 2010
39 patientswith male subfertility(51% isolatedteratozoospermia(WHO 1999)).Halo testRaw semeneAfterdensity gradientAfter Zech-selectorEbner et al, 2011
Page 1 and 2: Relevance of Sperm DNAFragmentation
Page 3: 1. Sperm DNA peculiarityDuring sper
Page 6 and 7: In mature sperm the DNA is• 85% p
Page 8: 2. Etiology of DNA Damagethe etiolo
Page 11 and 12: ROS• Small levels are essential f
Page 13 and 14: 3. DNA Fragmentation TestsDFI: DNA
Page 15 and 16: TUNEL assayLabels single and double
Page 17 and 18: SCD or Halo TestThe SCD is based on
Page 20 and 21: 4. Influence of lab procedures
Page 23: The potential detrimental effect of
Page 26 and 27: 90% of patients showed no strandbre
Page 30 and 31: DFImotilityRaw semen 15.8 ± 7.8% 3
Page 32 and 33: Why do fast progressive sperms show
Page 34 and 35: 5. Is DFI testing relevant forferti
Page 36 and 37: Fertility potential, threshold leve
Page 38 and 39: • in the general population:1-4%
Page 43 and 44: 23 papers that investigated the inf
Page 46 and 47: CONCLUSIONSsperm DNA damage is asso
Page 48 and 49: When oocytes from infertile patient
Page 50 and 51: Conclusions II• Small but signifi
Page 53 and 54: (infertile patients!)Infertile Pati

Relevance of Sperm DNA Fragmentation - eshre

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