c. In a private termination action, petitioner’s failure to include a prior custodyorder with the petition and failure to include the name and address of anappointed guardian rendered the petition fatally defective. In re Z.T.B., 170N.C. App. 564 (2005).5. Facts sufficient to support a determination that one or more grounds forterminating parental rights exist.a. The court cannot adjudicate a ground that is not alleged in the petition. See,e.g., In re B.L.H., 190 N.C. App. 142, aff'd per curiam 362 N.C. 674 (2008)(holding that a petition could not be amended to conform to the evidence andallege a new ground); In re S.R.G., 195 N.C. App. 79 (2009); In re C.W., 182N.C. App. 214 (2007). Cf. In re A.H., 183 N.C. App. 609 (2007) (holding thatalthough the petition did not specifically reference G.S. 7B-1111(a)(6), theallegations gave respondent sufficient notice that termination would be soughton the ground that she was incapable of providing proper care and supervisionof child).b. Although a motion [which the opinion refers to as a petition] asserted onlybarebones legal bases as grounds for terminating parental rights, it wassufficiently detailed because it incorporated by reference the entire juvenilefile in the matter. In re H.T., 180 N.C. App. 611 (2006).c. A bare allegation that the parent neglected the child and willfully abandonedthe child for six months did not comply with this pleading requirement, but anattached custody decree incorporated into the petition did contain sufficientfacts. In re Quevedo, 106 N.C. App. 574 (1992).d. Allegations need not be exhaustive or extensive, but they must put a party onnotice as to acts, omissions, or conditions that are at issue and must do morethan recite the statutory wording of the ground. In re Hardesty, 150 N.C. App.380 (2002). See also In re Humphrey, 156 N.C. App. 533 (2003) (holding thatthe allegations were sufficient to put respondent on notice even though thepetition did not specifically allege neglect).6. A statement that the petition or motion has not been filed to circumvent theUniform Child Custody Jurisdiction and Enforcement Act (UCCJEA).a. While the petition should include this statement, its omission did not result inprejudice to respondent and was not reversible error. In re Humphrey, 156N.C. App. 533 (2003). See also In re B.D., 174 N.C. App. 234 (2005); In reJ.D.S., 170 N.C. App. 244 (2005) (holding that omission of the statement didnot deprive the trial court of jurisdiction).September 2012TERMINATION OF PARENTAL RIGHTS© 2012 School of Government. The University of North Carolina at Chapel Hill25
Page 27$ blastdb_aliastool -dblist "nematode_mrna nematode_genomic" -dbtype nucl \-out nematode_all -title "Nematode RefSeq mRNA + Genomic"<strong>BLAST</strong> Help <strong>BLAST</strong> Help <strong>BLAST</strong> Help <strong>BLAST</strong> Help5.7.2 Create a subset of a <strong>BLAST</strong> databaseThe nematode_mrna database contains RefSeq mRNAs for several species of round worms.The best subset is from C. elegance. In most cases, we want to search this subset instead of thecomplete collection. Since the database entries are from NCBI nucleotide databases and thedatabase is formatted with ”-parse_seqids”, we can use the “-gilist c_elegance_mrna.gi”parameter/value pair to limit the search to the subset of interest, alternatively, we can create asubset of the nematode_mrna database as follows:$ blastdb_aliastool -db nematode_mrna -gilist c_elegance_mrna.gi -dbtype \nucl -out c_elegance_mrna -title "C. elegans refseq mRNA entries"Note: one can also specify multiple databases using the -db parameter of blastdb_aliastool.5.8 Reformat <strong>BLAST</strong> reports with blast_formatterIt may be helpful to view the same <strong>BLAST</strong> results in different formats. A user may first parsethe tabular format looking for matches meeting a certain criteria, then go back and examinethe relevant alignments in the full <strong>BLAST</strong> report. He may also first look at pair-wisealignments, then decide to use a query-anchored view. Viewing a <strong>BLAST</strong> report in differentformats has been possible on the NCBI <strong>BLAST</strong> web site since 2000, but has not been possiblewith stand-alone <strong>BLAST</strong> runs. The blast_formatter allows this, if the original search producedblast archive format using the –outfmt 11 switch. The query sequence, the <strong>BLAST</strong> options,the masking information, the name of the database, and the alignment are written out as ASN.1 (a structured format similar to XML). The blast_formatter reads this information and formatsa report. The <strong>BLAST</strong> database used for the original search must be available, or the sequencesneed to be fetched from the NCBI, assuming the database contains sequences in the publicdataset. The box below illustrates the procedure. A blastn run first produces the <strong>BLAST</strong> archiveformat, and the blast_fomatter then reads the file and produces tabular output.Blast_formatter will format stand-alone searches performed with an earlier version of adatabase if both the search and formatting databases are prepared so that fetching by sequenceID is possible. To enable fetching by sequence ID use the –parse_seqids flag when runningmakeblastdb, or (if available) download preformatted <strong>BLAST</strong> databases from ftp://ftp.ncbi.nlm.nih.gov/blast/db/ using update_blastdb.pl (provided as part of the <strong>BLAST</strong>+package). Currently the blast archive format and blast_formatter do not work with databasefree searches (i.e., -subject rather than –db was used for the original search).$ echo 1786181 | blastn -db ecoli -outfmt 11 -out out.1786181.asn$ blast_formatter -archive out.1786181.asn -outfmt "7 qacc sacc evalueqstart qend sstart send"# <strong>BLAST</strong>N 2.2.24+# Query: gi|1786181|gb|AE000111.1|AE000111 Escherichia coli K-12 MG1655section 1 of 400# Database: ecoli# Fields: query acc., subject acc., evalue, q. start, q. end,s. start, s. end# 85 hits foundAE000111 AE000111 0.0 1 10596 1 10596AE000111 AE000174 8e-30 5565 5671 6928 6821AE000111 AE000394 1e-27 5587 5671 135 219<strong>BLAST</strong> <strong>Command</strong> <strong>Line</strong> <strong>Applications</strong> <strong>User</strong> <strong>Manual</strong>