Etudes sur le mécanisme de remodelage des nucléosomes par ...

tel-00413908, version 1 - 7 Sep 2009
IV.2.3 The base of H2A docking domain is essential for SWI/SNF mediated
mobilization of nucleosomes………………………………………...………141
IV.2.4 RSC is more sensitive to perturbations in the docking domain of H2A
……………………………………………………………………………….144
IV.2.5 Generation of distinct remodeled forms by SWI/SNF on H2A.Bbd
nucleosomes…………………………………………………………………146
IV.2.6 The docking domain of H2A.Bbd is responsible for anomalous
remodeling by SWI/SNF…………………………………………………….149
I V.2.7 R SC mediated r e modeling i s s i milar t o S WI/SNF o n
H2A.dockingdomain.Bbd nucleosomes……………………………………..151
IV.3 Discussion………………………………………………………………….......152
IV.4 Experimental procedures
IV.4.1 Preparation of DNA probes…………………………………………..154
IV.4.2 Proteins……………………………………………………………….154
IV.4.3 Nucleosome reconstitutions………………………………………….154
IV.4.4 DNaseI and hydroxyl radical footprinting…………………………...155
IV.4.5 Nucleosome sliding assay……………………………………………155
IV.4.6 AFM analysis…………………………...…………………………….156
IV.4.7 One pot restriction assay……………………………………………..156
IV.4.8 Supplementary information for Chapter II, III and IV……………….157
V. General conclusions and perspective…………………………………………159
BIBLIOGRAPHY..................................................................................................................163
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