Contents - Medicinski Fakultet u Sarajevu - University of Sarajevo

Oral presentationCHROMOGRANINE A IMMUNOREACTIVITY IN THE MYENTERICPLEXUS OF THE HUMAN FETAL DUODENUM IN THE THIRD ANDTHE FIFTH MONTH OF DEVELOPMENTNikolić I 1 , Todorović V 2 , Petrović V 1 , Mitić D 31Institute for histology and embryology,Medical faculty, University in Nis2Institute for biomedical researchesof the University of Belgrade3Clinic for gynecology and obstetrics,Clinical center of Nis, SerbiaE-mail: inikolic@ni.ac.rsIntroduction: Myenteric plexus (MP) is part of the entericnervous system and is localized in the muscularisof the intestines. CgA immunoreactivity was examinedin MP of the human fetus duodenum in the third andthe fifth month of the development.Material and methods: As a material we used 11 duodenumsin the third and the fifth month of the gestation.All the duodenums were embedded in the paraffinand the tissue samples were stained by using immunohistochemicalPAP technique for identification of CgA. Wedetermined volume fraction of MP in the muscular layer.Results our study show that CgA-immunoreactivity ispresent in the ganglia of MP in the fetal duodenum inthe third and the fifth month of the development. MP inthe third month of the development is thin and showsweak CgA-immunoreactivity. In the fifth month MP ismore voluminous with strong CgA-immunoreactivity.By comparing the obtained values for the volume fractionof MP in the muscular layer between the groups,we found that there is statistically significant differencebetween the groups.Conclusion: The obtained results show that the developmentof the enteric nervous system is tightly connectedand is in accordance with the functional differentiationof all the structures in the gastrointestinalsystem that takes place in 10. and 18. week of the development.Keywords: chromogranine A, fetal duodenum, myentericplexus35