BioDocAnalyze (BDA) digital - Biometra

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20Image Capture DSLR3.2.2 The Card „Settings“Shooting Mode For display of capture mode (set at the camera). Recommended mode is„M“ (manual).- M Exposure time and lens aperture can be set in lines „Av“ and „Tv“. Evenfor the use of profile parameters the mode should be „M“.- P The camera sets the capture parameters exposure time Tv and lensaperture Av automatically.When the photo is taken the automatically used parameters are displayedon card “Shot Conditions”.- Tv The camera automatically chooses the optimal lens aperture Av inrelation to the manually set exposure time. After the picture is taken theautomatically used aperture is displayed in the card Shot Conditions.- Av The camera automatically chooses the optimal exposure time Tv inrelation to the manually set lens aperture. After the picture is taken theautomatically used exposure time is displayed in the card Shot Conditions.Lens aperture,AvExposure time,TvResolutionFor setting the lens aperture. A small aperture number stands for a wideopen lens, a high aperture number for a more closed lens. The depth offocus increases with rising aperture number (= smaller lens aperture). Agarosegels, especially thicker gels, should be photographed with high aperturenumber (= small aperture). Otherwise photos may not be focussed.The largest aperture (= small number) available depends on the zoom factorset. With zoom 55 mm the largest aperture is 5.65, with zoom 35 aperture4 and with zoom 18 the largest possible aperture to be set is 3.5.For setting the exposure time. The time is to be set in dependence ofsample/illumination and lens aperture. For smaller apertures (= high aperturenumber, e.g. 8) exposure times of up to 8 seconds can be necessaryfor a photo of fluorescent gels.For choice of image resolution in pixels. The higher the resolution thebigger the file size.High resolutions will cause longer data transfer times from camera to PCon computers with slow processors and memory below 1 GB RAM. For anefficient operating it is recommended to use a computer with at least 2 GBRAM and a 2.8 GHz processor.BDAdigital Manual, Vers. 02_12
Large + FineMedium + FineSmall + FineSmall S2ISOExposureCompensationEOS 1100DResolution (pixels)Approx. 12.2 MPApprox. 6.3 MPApprox. 3.4 MPApprox. 2.5 MPCompressionFineFineFineFine21Image Capture DSLRFor setting of the sensitivity.In most cases it is advised to use ISO 100. It provides sufficient sensitivityby lowest background noise. Only for the acquisition of very faint signals athigh zoom factors it can be necessary to raise the sensitivity to higher ISOscores.For setting an exposure correction. A fine-tuning of the optimal exposurecan be done in small steps. This function is not available in shooting mode„M“ because lens aperture and exposure time are specifically set in themanual mode.3.2.3 Camera Settings for Typical GelsThe software provides camera settings for two typical gel types in Profiles. Starting withthese settings it is very easy and fast to optimize the camera settings for individual gels.Gel typeEtBr Stained Agarose Gelon UV tableProfile „DNA/EtBr“Shooting Mode M MLens aperture Av 5.6 5.6Exposure time Tv 8 s 1/50 sISO 100 100Coloured PolyacrylamideGelon UV table with yellowconverter plate, without UVfilter on camera lens,e.g. silver or CoomassieBlue stained polyacrylamidegelProfile „Converter“Resolution Medium + Fine Medium + FineBDAdigital Manual, Vers. 02_12
Page 1 and 2: Gel Documentation SystemBioDocAnaly
Page 3 and 4: Contents3Contents1 INSTALLATION OF
Page 5 and 6: 1.2.3 Set up of single lens reflex
Page 7 and 8: 7Installation1.2.3.3 Attaching the
Page 9 and 10: 9Installation2. Covering of lens re
Page 12 and 13: 12Installation1.3 Installation of t
Page 14 and 15: 14InstallationSetting the Shooting
Page 16 and 17: 16Start of Program2 Start of the Pr
Page 18 and 19: 18Image Capture DSLRCameraIn case t
Page 22 and 23: 22Image Capture DSLR3.2.4 Further F
Page 24 and 25: 24Image Capture DSLR3.2.6 Troublesh
Page 26 and 27: 26Image Capture Compact Camera4 BDA
Page 28 and 29: 28Image Capture Compact Camera4.2 F
Page 30 and 31: 30Image Capture Compact Camera4.2.3
Page 32 and 33: 32Image Capture Compact Camera4.2.5
Page 34 and 35: 34Image Capture Compact CameraAnaly
Page 36 and 37: 36Service6 Equipment Decontaminatio
Page 38: 38Service7 DisposalDue to the appli

BioDocAnalyze (BDA) digital - Biometra

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