Qingzhumeasured with 1 h intervals using automatic record-meter inserted in the four bins.Temperature <strong>of</strong> the cold chamber was also monitored. The liquid ice was supplied byOptimar (Company, in Iceland) with initial salt content <strong>of</strong> 3.5% and ice content <strong>of</strong>27%~30%. The flack ice was made <strong>of</strong> potable water at the laboratory.The ratio <strong>of</strong> ice to shrimp had been theoretically calculated taking into account how muchice was needed to chill the shrimp down from 4-5°C to 0°C and how much ice to keep theshrimp chilled for 10 days (Table 1). The mass <strong>of</strong> ice used (M i used) was 5-6 times thecalculated minimum value the mass (M i total) determined theoretically. This was done tomake sure that there would be enough ice for the during the whole storage period.Table 1: Latent heat <strong>of</strong> fusion (∆H f ) <strong>of</strong> the different cooling agent, the minimumquantity needed to cool the shrimp and keep it chilled (Mi total) and the quantityused for 15 kg <strong>of</strong> shrimp (Mi used).Type <strong>of</strong>cooling agentMs * Cps * ∆T Ratio <strong>of</strong> ice(kg)*(kcal/kg°C)*(°C) (%)∆Hf(kcal/kg)Mc for chilling(kg)Mc for storage(kg)Mi total(kg)Flake ice 15*0,8*10=120 100 80 1,5 2,3 3,8 15,0Liquid ice 15*0,8*10=120 30 24 5,0 7,5 12,5 43,5Mi used(kg)Salt-water +ice15*0,8*10=120 70 56 2,1 3,2 5,4 22,5M c for chilling (from 10 to 0°C) = ( M s * Cp s * ∆T) / H f = (15*0,762*(20-0))/80,M c for storage = (1,5%*M s *10 days * 80kcal/kg) * ratio <strong>of</strong> ice in the cooling agent,M c = mass <strong>of</strong> cooling agent (kg), M s = mass <strong>of</strong> shrimp (15 kg),Cp s = specific heat used for shrimp (80 kcal/kg), ∆T = 10°C, ∆H f = latent heat <strong>of</strong> fusionOn days 0, 1, 4 and 6 <strong>of</strong> storage, corresponding to days 3, 4, 7 and 9 after catch, duplicatesamples were taken from each lot <strong>of</strong> the four different groups <strong>of</strong> shrimp stored in thedifferent conditions. The samples were submitted to microbiological, chemical, physicaland sensory analysis.Table 2: Experimental groups and sampling plan.Group Type <strong>of</strong> ice Ratio <strong>of</strong> Draining Storage temp. Sampling daysshrimp to ice during storage 0 1 4 6ICE/+ Flake ice 1:1.5 Yes 1.5±0.4 o C Day0 ICE/+1 ICE/+4 ICE/+6LIQ/+ Liquid ice 1:2.9 Yes 1.5±0.4 o C Day0 LIQ/+1 LIQ/+4 LIQ/+6S-ICE/- Salt-water (30%)+ ice (70%) 1:1.5 No -1.5±0.3 o C Day0 S-ICE/-1 S-ICE/-4 S-ICE/-6LIQ/- Liquid ice 1:2.9 No -1.5±0.3 o C Day0 LIQ/-1 LIQ/-4 LIQ/-6UNU-Fisheries Training Programme 12
Qingzhu3.2 Sensory evaluationA <strong>Quality</strong> Grading Scheme was used to evaluate the quality <strong>of</strong> whole shrimp (Table 3).Duplicate samples from each <strong>of</strong> the four storage conditions were taken at regular intervals(on days 0, 1, 4 and 6 <strong>of</strong> storage) for each group and placed in two clean transparent glasscontainers, after 20 min the assessment was carried out under room temperature andadequate fluorescent light. The samples were coded with a random three digit number.The panalists were not aware <strong>of</strong> the number <strong>of</strong> storage days <strong>of</strong> the shrimp and did notknow which two containers were the same group prior to assessment. The panelconstituted <strong>of</strong> eight members who had been trained in evaluating quality <strong>of</strong> shrimp andthe characteristic sensory attributes.Table 3: Score sheet for quality grading scheme <strong>of</strong> whole shrimp (IFL 2003).Score / GradingDescription5 Excellent Colour is dark red to bright pink. Roes are blue-green (copper). Strong seaweedy,marine odour. Strong sweet shrimp taste.4 Good Colour is natural light pink. Roes are blue-green (copper). Weak characteristicshrimp odour. Weak sweet shrimp taste.3 Moderate Marine/shrimp odour is diminishing, weak “fishy odour”, even slight ammonia.Colour is natural light pink with grey-greenish or yellowish discoloration.Roes are light green. Taste is natural not sweet to weak “fishy taste”.2 Borderline----Clearly not FreshWeak ammonia odour. Colour is natural light pink with grey-greenish oryellowish discoloration. Roes are discoloured. Blackening on the head canbe spotted. Distinct fishy taste with bitter aftertaste.1 Unfit Spoiled Ammonia odour. Colour is natural light pink with grey-greenish or yellowishdiscoloration. Roes are Dark. The blackening on the head is extensive.Spoiled, taste with strong, bitter aftertaste.3.3 Protein measurementProtein content in shrimp meat was determined by the Kjeldahl method (ISO 1997). Asample <strong>of</strong> 5.00 g was digested in sulphuric acid in presence <strong>of</strong> copper as a catalyst.Thereafter, the sample was placed in distillation unit, 2400 Kjeltec Auto Sample System.The acid solution was made alkaline by a sodium hydroxide solution. The ammonia wasdistilled into boric acid and the acid was simultaneously titrated with diluted H 2 SO 4 . Thenitrogen content was multiplied by the factor 6.25 to get the ratio <strong>of</strong> crude protein.UNU-Fisheries Training Programme 13
- Page 1 and 2: Final Project 2003Quality Indicator
- Page 3 and 4: QingzhuACKNOWLEDGEMENTS............
- Page 5 and 6: Qingzhu1 INTRODUCTIONThe northern s
- Page 8: Qingzhu2.3 Assessment methods of fr
- Page 11: Qingzhurecovered its height during
- Page 15 and 16: Qingzhu3.9 pH measurementpH was mea
- Page 17 and 18: Qingzhu3.13 Data analysisThe data,
- Page 19 and 20: QingzhuThe lowest score was awarded
- Page 21 and 22: QingzhuTVB-N mgN/100g12010080604020
- Page 23 and 24: Qingzhu4.5 Water contentThe effects
- Page 25 and 26: Qingzhu4.7 Water-holding capacity (
- Page 27 and 28: QingzhuSpringiness (N)0.80.70.60.50
- Page 29 and 30: Qingzhu250S-ICE/-200Current (nA) --
- Page 31 and 32: QingzhuTable 4: Correlation (r) bet
- Page 33 and 34: Qingzhu1.0PC2Bi-plotResilience Cohe
- Page 35 and 36: Qingzhuof the main spoilage-causing
- Page 37 and 38: Qingzhu5.6 Correlation analysisThe
- Page 39 and 40: QingzhuNH 3 response of electronic
- Page 41 and 42: QingzhuLIST OF REFERENCESAitken, A.
- Page 43 and 44: Qingzhuof fish in research and indu
- Page 45 and 46: QingzhuAPPENDIXAnalysis of Variance
- Page 47 and 48: QingzhuICE/+4 5 8,564 0,6168125 7,0
- Page 49 and 50: QingzhuTotal (Adjusted) 31 18,5293T
- Page 51 and 52: QingzhuGroup Count Mean Different F
- Page 53 and 54: QingzhuAll 24 0,345750,0572743A: Gr
- Page 55 and 56: QingzhuSource Sum of Mean Prob Powe
- Page 57 and 58: QingzhuS-ICE/-6 8 1,0625 0,12734960
- Page 59 and 60: QingzhuTerm DF Squares Square F-Rat
- Page 61 and 62: QingzhuGroup Count Mean Different F
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QingzhuTerm DF Squares Square F-Rat