Artemisia thuscula Cav.: antibacterial, antifungal activity of theplant extracts <strong>and</strong> associated endophytesCosoveanu Andreea *1 , Da Silva, Evel<strong>in</strong> 2 , Gimenez Mariño, Crist<strong>in</strong>a. 2 , Núñez Trujillo, Garoe 2 ,González-Coloma, Azucena 3 , Frias Viera I. 2 , Cabrera, R. 21 .- University of Agricultural Sciences <strong>and</strong> Veter<strong>in</strong>ary Medic<strong>in</strong>e (USAMV), Faculty of Horticulture (Bucharest);2 University of La Laguna (ULL), Faculty of Biology, UDI Phytopathology (Tenerife); 3.- ICA- CSIC- (Madrid)*Correspond<strong>in</strong>g author: <strong>and</strong>reeacosoveanu@gmail.comAbstract In this paper we are present<strong>in</strong>g prelim<strong>in</strong>ary results for theantifungal <strong>and</strong> antibacterian activity of the Artemisia thuscula Cav. alltogether with the endophytic communities encountered <strong>in</strong> symbiosis with thisspecie. This plant is endemic for the <strong>Canary</strong> <strong>Isl<strong>and</strong>s</strong> <strong>and</strong> it is recognised for itstraditional medic<strong>in</strong>al use (like other species of the same genus <strong>in</strong> the rest ofthe world) <strong>and</strong> for be<strong>in</strong>g a functional repellent of <strong>in</strong>sects.The ethanol extracts tested showed an <strong>in</strong>terest<strong>in</strong>g activity aga<strong>in</strong>st thephytopathogenic <strong>fungi</strong> Fusarium monilforme, F. solani <strong>and</strong> F. oxysporum <strong>and</strong>antibiotic activity aga<strong>in</strong>st 2 Gram-positive bacteria: Bacillus cereus <strong>and</strong>Streptomyces griseus, <strong>in</strong> an primary screen<strong>in</strong>g.The diversity of endophytes found <strong>in</strong> this plant, especially <strong>in</strong> the roots, showedpromis<strong>in</strong>g results support<strong>in</strong>g further work on this species.Key wordsArtemisiaantifungalantibacterialendophytesthuscula,activity,activity,Experimental results concern<strong>in</strong>g the effect of sucroseconcentration on cell biomass <strong>and</strong> synthesized anthocyan<strong>in</strong>amount <strong>in</strong> the callus cultureLazăr A. 1* , Petolescu Cerasela 11 Banat‟s University of Agricultural Sciences <strong>and</strong> Veter<strong>in</strong>ary Medic<strong>in</strong>e Timisoara, Faculty of Horticulture <strong>and</strong>Forestry*Correspond<strong>in</strong>g author. Email: alexlazar2002@yahoo.comAbstract The growth rate of cell biomass registered maximum meanvalue <strong>in</strong> the cultivation <strong>in</strong>terval of 24 days for Burgund Mare, CabernetSauvignon <strong>and</strong> P<strong>in</strong>ot Noir <strong>and</strong> 27 days for Negru T<strong>in</strong>ctorial, Oporto <strong>and</strong>Cabernet Sauvignon varieties. Regard<strong>in</strong>g the sucrose concentration added<strong>in</strong>to the culture medium, it has been observed that callus growth rateregistered for the concentration of 30 g/l <strong>and</strong> the most reduced values forcallus growth rate were registered for the sucrose concentration of 60 g/l.Compar<strong>in</strong>g the mean obta<strong>in</strong>ed values for the studied grapev<strong>in</strong>e varieties, ithas been observed that the largest values for synthesized anthocyan<strong>in</strong>s <strong>in</strong>callus culture has been registered for Negru T<strong>in</strong>ctorial variety followed byBurgund Mare, Oporto, Merlot, P<strong>in</strong>ot Noir <strong>and</strong> Cabernet Sauvignon. Thesame hierarchy is ma<strong>in</strong>ta<strong>in</strong>ed for behaviour of grapev<strong>in</strong>e varieties <strong>in</strong> terms ofeach type of sucrose concentration added <strong>in</strong>to culture media. The differencesfound between varieties depend<strong>in</strong>g on anthocyan<strong>in</strong> amount <strong>from</strong> calluscultures were very significant <strong>in</strong> most cases.Key wordscallus culture, cell biomass,anthocyan<strong>in</strong>, <strong>Vitis</strong> <strong>v<strong>in</strong>ifera</strong> L.
Experimental results concern<strong>in</strong>g the effect of photoperiod <strong>and</strong>callus culture duration on anthocyan<strong>in</strong> synthesisLazăr A. 1* , Petolescu Cerasela 11 Banat‟s University of Agricultural Sciences <strong>and</strong> Veter<strong>in</strong>ary Medic<strong>in</strong>e Timisoara, Faculty of Horticulture <strong>and</strong>Forestry*Correspond<strong>in</strong>g author. Email: alexlazar2002@yahoo.comAbstract Light has a stimulat<strong>in</strong>g effect on callus biosynthetic potential, thegenotype is the ma<strong>in</strong> factor that controls the productive potential of cultures.In photoperiod conditions, the highest biosynthetic potential have beenobserved for calli of Negru T<strong>in</strong>ctorial (12,04 mg/g) <strong>and</strong> Burgund Mare (5,88mg/g) varieties, while the lowest for calli ris<strong>in</strong>g <strong>from</strong> Cabernet Sauvignon (1,14mg/g), P<strong>in</strong>ot Noir (2,76 mg/g) <strong>and</strong> Merlot (3,17 mg/g) varieties.Key wordscallus culture, cell biomass,anthocyan<strong>in</strong>, <strong>Vitis</strong> <strong>v<strong>in</strong>ifera</strong> L.Impact of biostimulators <strong>and</strong> of root<strong>in</strong>g substratum on sapl<strong>in</strong>ggrowth <strong>in</strong> Ficus carica L.Poşta Daniela Sab<strong>in</strong>a 1*1 Banat‟s University of Agricultural Sciences <strong>and</strong> Veter<strong>in</strong>ary Medic<strong>in</strong>e <strong>from</strong> Timisoara, Faculty of Horticulture<strong>and</strong> Forestry*Correspond<strong>in</strong>g author. Email: posta.daniela@gmail.comAbstract The biological material is represented by sapl<strong>in</strong>gs of Ficuscarica L. obta<strong>in</strong>ed vegetatively through cutt<strong>in</strong>gs us<strong>in</strong>g lignified cuts.The root<strong>in</strong>g substratum used <strong>in</strong> the experiment was made up withwell-washed river s<strong>and</strong>.The root<strong>in</strong>g biostimulators we used were: a solution of Atonik,1:4,000, i.e. 0.25 ml solution per 1 l of water, <strong>and</strong> Radistim 2 powder.We made morphological feature biometric measurements <strong>in</strong> eachsapl<strong>in</strong>g: sapl<strong>in</strong>g height, diameter of the collar, diameter of root system,number of roots, <strong>and</strong> root lengthKey wordsFicus carica L., cutt<strong>in</strong>gs,biostimulators, root<strong>in</strong>gsubstrate nutrient