Endophytic fungi from Vitis vinifera L. isolated in Canary Islands and ...

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ice to avoid any shortcomings related to improper handling. Both tubes andmicropipette tips to have been sterilized by autoclaving.Only five of the 10 RAPD primers tested generated bands toregenerate ALBITZIA LEBECK genotype OPA-09, OPA-13, OPA-17, OPC-11, OPC-14. For Albizzia julibrisin was tested 10 RAPD primers and only 5has generated bands.Production of virus free potato plantletsDanci M., 1* Danci Oana 1 , Mike Luiza 2 , Baciu Anca 2 , Olaru Daniela 1 , Petolescu Cerasela 1 ,Berbentea F. 1 , David I. 11 Banat‟s University of Agricultural Sciences and Veterinary Medicine Timişoara; 2 Potato Research andDevelopment Station – Targu Secuiesc, Romania*Corresponding author. Email: marcel_research@yahoo.comAbstract Potatoes a high susceptibility to viral, bacterial and fungaldiseases impose production of virus free material for healthy seed productionsince plant viruses are pathogens that cannot be destroyed by using plantprotection chemicals. Meristem tip culture technique is one of the most usedfor potato in vitro culture initiation and is most of all used in order to obtainvirus free plantlets. For this study six new Romanian potato cultivars wereused. Explants were prepared for tissue culture and half of them underwentchemical (using the antiviral chemical-ribavirin) treatement. Culture tubeswere incubated at 20-21 o C in 16 hr photoperiod with 2000 lux light intensityfor 6-8 weeks. Leaf samples of 10 plantlets of each cultivar were tested forviruses: PVS, PVY, PVX and PLRV by ELISA technique. Virus-freeindividuals were measured for several biometrical parameters andsubcultured on fresh multiplication media for 4 weeks. Best virus freeproduction was obtained after Ribavirin treatment even if regenerationcapacity after chemical therapy decreased with around 10%.Key wordsmeristem tip culture, virusfreeplantlets regeneration,virus indexing, Solanumtuberosum L., RibavirinEvaluation of some indirect indices to identify droughttolerance in barleyVelicevici Giancarla 1 *, Madosă E. 1 , Şumălan R. 1 , Ciulca Adriana 1 , Petolescu Cerasela 11 University of Agricultural Sciences and Veterinary Medicine of Banat Timisoara, Faculty of Horticulture andForestry, Calea Aradului 119, 300645, Romania*Corresponding author. Email: giancarlavely2000@yahoo.comAbstract The obiective of this study was to evaluate the ability of severalindices to identify drought tolerance of some hybrids in laboratory conditions.Germination stress index (GSI), Promptness index in stress (PIS) and nonstress (PINS) conditions were recordered in the controlled conditions oflaboratory under two different stress and non stress water regime. Theosmotic stress were induced by PEG 6000 solution with the osmotic potential( 0 Bars, -7.35 Bars) using method suggested by Kaufman, and replicatedthree time, at a temperature of 22±2 o C. Therefore, drought induced by usingphysiological germination solutions with a different osmotic pressure levelsdirectly influence the germination of the seeding material. These indices maybe screened for indirect selection of drought tolerance in the initial stage ofthe crop growth.Key wordsStress tolerance index,germination stress index,barley
Influence of Hydric Deficit on Root Growth Dynamics in SomeGenotypes of Barley (Hordeum Vulgare)Velicevici Giancarla 1* , Sumalan R. 1 , Madosa E. 1 , Ciulca Elena Adriana 1 , Bitea Nicoleta 1 ,Malaescu Mihaela 11 University of Agricultural Sciences and Veterinary Medicine of Banat Timisoara, Faculty of Agriculture andForestry, Calea Aradului 119, Romania;*Corresponding author. Email: giancarlavely2000@yahoo.comAbstract Drought is one of the most important types of abiotic stress thataffects stability and amount of yield. This study was conducted to screen fordrought tolerance at early seedling stages for 19 Romanian and foreign winterbarley cultivars. The objective of this study was to determine if there weredifferential responses to water deficit on root growth dynamics in some barleycultivars. Fluid deficit was induced by polyethylene-glycol (PEG6000) solution,using control and 3 variants with different osmotic pressure (-2.72 Bars, -4.48Bars, -7.35 Bars). Determinations of root growth were efectuate after periodsof 5/10/15/ 20 and 25 days from the induction of osmotic stress. It can beobserved that the varieties, seedlings age or treatment with PEG hadsignificantly positive influence on the roots growth in barley genotypesstudied. The age of seedlings presented the highest influence on thevariability of this character. The best results in terms of root growth wereobtained for the Regal and Andrei variety these being found to be lessinfluenced by PEG's action, the varieties most affected were Adi, Plaisant,and DH261/22, DH260/18 DH 254/10 lines.Key wordsbarley, drought tolerance,root growth, Polyethyleneglycol6000Researches concerning pollen’s germination of some grapevine varieties in conditions of D.S. TimisoaraBlidariu C. 1* , Dobrei A. 11 Banat‟s University of Agricultural Sciences and Veterinary Medicine Timisoara, Faculty of Horticulture andForestry*Corresponding author. Email: ciprian_blidariu@yahoo.comAbstract Grape vine‟s culture was of great importance not only forancient people form different areas, but also for our ancestors. Out ofresearches done it results that grape vine was cultivated on the territory of ourcountry even since 700-1000 years B.C.According to FAO 2010 data the area cultivated in Romania in 2009with grape vines was of 183814 ha, being on the 11 th place in the world.In this article we made some observations upon Burgund, MuscatOttonel, Muscat Hamburg and Coarnă neagră grape varieties, which behavedifferently concerning the flowering process.The research made pointed out a good flowering process and pollination forBurgund and Muscat Ottonel and differentiated ones for Muscat Hamburg andCoarnă neagră varieties.Key wordsgrape vine, germination,grains, pollen, fertility
Page 1: JOURNAL of Horticulture, Forestry a
Page 4 and 5: Gared variety - result of potato br
Page 6 and 7: Effects of appling EU legislation o
Page 8 and 9: flax and hemp, or which are threate
Page 10 and 11: Artemisia thuscula Cav.: antibacter
Page 12 and 13: Research concerning the production
Page 14 and 15: Variability analysis of grain yield
Page 16 and 17: The effect of genotype and water st
Page 18 and 19: Predators of Pityogenes chalcograph
Page 20 and 21: The evolution of the growth and fru
Page 22 and 23: Aspects regarding the physiology of
Page 26 and 27: Influence of organic and mineral fe
Page 28 and 29: during summer. In order to obtain s
Page 30 and 31: Measures for sustainable management

Endophytic fungi from Vitis vinifera L. isolated in Canary Islands and ...

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