p/ace™ mdq detectors - Beckman Coulter

CE APPLICATION KITSC HIRAL A NALYSIS ( CONTINUED)If no resolution is obtained from the primary strategy, Beckman Coulter offers a secondary approach with the P/ACE MDQChiral Methods Development Kit. This kit utilizes a selection of four non-charged cyclodextrins.C HIRAL M ETHODS D EVELOPMENT S ECONDARY S TRATEGYChoose UV wavelengthChoose CD typeor try all 4 CDsSeparate with:High pH BufferLow and High [CD]Reversed PolarityACIDSolute: Acid or BaseBASESeparate with:Low pH BufferLow and High [CD]Normal PolarityChiral Separation?NDetermine pK of soluteNChiral Separation?YYSeparate with:pH = pK BufferLow and High [CS]Acids: Reversed PolarityBases: Normal PolarityChiral Separation?NTry other Chiral SelectorsYDetermine Optimize pK of solute[CD]; E; T; pHPART NUMBERDESCRIPTION501300 P/ACE MDQ Chiral Methods Development KitConsists of 2 neutral capillaries, high pH buffer, low pH buffer,adjustable pH buffer, β-cyclodextrin, γ-cyclodextrin, HP-β-cyclodextrin,and DM-β-cyclodextrin, Test Mix, Methods Development Guide, ChiralMethods Development Flow Chart, and Buffers Dilution Guide.Beckman CoulterContentsIndex25