CE APPLICATION KITSCEQ 2000XL APPLICATION K ITSPART NUMBERDESCRIPTION608000 CEQ DTCS KitDye-labeled dideoxy-terminator cycle sequencing kit for 96 reactions.Includes: thermostable DNA polymerase and pyrophosphatase;CEQ dye-labeled terminators (ddUTP, ddGTP, ddCTP, ddATP);dNTP mix solution; sequencing reaction buffer, pUC18 controltemplate, M13(-47) sequencing primer, mineral oil and glycogen.Storage temperature: -20°C.608120 CEQ DTCS – Quick Start KitDye terminator cycle sequencing (DTCS) reactions incorporate manycomponents including DNA polymerase, pyrophosphatase buffer,dNTPs, dye terminators, DNA templates and primers. The CEQ DTCSQuick Start Kit simplifies this process by using a master-mix of many ofthe components—reducing the number of pipetting steps from 10 to 4while using larger transfer volumes to reduce pipetting error. Each kitprovides enough reagents to complete 100 sequencing reactions.608010 CEQ LFR Gel ISpecial CEQ polyacrylamide denaturing gel assures high-resolutionseparations on the CEQ 2000XL. Used for both DNA sequencing andfragment analysis. Packaged in a cartridge for quick reloading byoperator. The cartridge holds 11.5 mL of polyacrylamide gel,sufficient for 12 loads into capillary arrays (96 separations).Storage temperature: 6°C.608012 CEQ Separation Buffer (quantity 4/pack)Each bottle has a twist top with a convenient dropper tip and containssufficient buffer (30 mL) to fill one flat-bottomed 96-well CEQ 2000XLBuffer Plate. Storage temperature: 6°C.608098 CEQ DNA Size Standard Kit– 400DNA size standard for analysis of fragments up to 400nucleotides.Sufficient for 96 fragment analysis separations. IncludesDNA fragments of the following sizes labeled with CEQ WellREDfluorescent dye: 60, 70, 80, 90, 100, 120, 140, 160, 180, 190, 200, 220,240, 260, 280, 300, 320, 340, 360, 380, 400, and 420 nucleotides.Storage temperature: -20°C. Includes mineral oil for overlay.608095 CEQ DNA Size Standard Kit– 600DNA size standard for analysis of fragments up to 600 nucleotides.Sufficient for 96 fragment analysis separations. Includes DNAfragments of the following sizes labeled with CEQ WellRED fluorescentdye: 60, 70, 80, 90, 100, 120, 140, 160, 180, 190, 200, 220, 240, 260,280, 300, 320, 340, 360, 380, 400, 420, 440, 460, 480, 500, 520, 540,560, 580, 600, 620, and 640 nucleotides. Storage temperature: -20°C.Includes mineral oil for overlay.608105 CEQ Fragment Analysis Test SampleA set of fluorescently labeled DNA fragments of known sizes and definedspacing used to verify the resolution power of the CEQ 2000XL fragmentanalysis system. Sufficient for 24 tests. Storage temperature: -20°C.<strong>Beckman</strong> <strong>Coulter</strong>ContentsIndex34
CE CHEMISTRY KITSB UFFERS,TEST M IXTURES, AND R EAGENTSPART NUMBERDESCRIPTION338437 Capillary Performance Test KitThe Capillary Performance Test Kit is designed to test functionality ofthe CE system. The test mixture consists of low concentrations of threeclosely related compounds (benzoic acid, p-hydroxybenzoic acid andp-hydroxyphenylacetic acid—see structures). Analyzing this testmixture confirms system resolution and sensitivity performance.The kit contains Test Mixture A (20 mL), Run Buffer A(50 mL), and Regenerator Solution A (20 mL). One kit is includedwith each P/ACE .338425 Capillary Performance Test Mixture ABenzoic acid, p-hydroxybenzoic acid, p-hydroxyphenylacetic acid(quantity 20 mL).0.016d:\ train\cedata~1\acids1, Channel AOHBA0.016338426 Capillary Performance Run Buffer A100 mM sodium borate, pH 8.3 (quantity 50 mL).0.014BA0.014338424 Capillary Regenerator Solution A0.1 N sodium hydroxide (quantity 50 mL).0.0120.010AU0.008PhenylAcet0.0120.0100.008AU501333 Capillary Performance Test Mix BThis test mix consists of two closely related compounds (P-OH benzoicacid and P-OH phenylacetic acid. Analyzing this test mixture confirmssystem resolution and sensitivity performance.0.0060.0040.0060.004338436 Borate Buffer KitIncludes boric acid solution, 0.4 M, 50 mL sodium tetraborate solution,0.1 M (quantity 50 mL).0.0020.002338433 Boric Acid, 0.4 M (quantity 50 mL)0.0003.0 3.5 4.0 4.5 5.0 5.5Minutes0.000338434 Sodium Tetraborate, 0.1 M(quantity 50 mL)p-OH Phenylacetic Acid p-OH Benzoic Acid Benzoic Acid<strong>Beckman</strong> <strong>Coulter</strong>ContentsIndex35