p/ace™ mdq detectors - Beckman Coulter

CE APPLICATION KITSN UCLEIC A CIDS ( CONTINUED)PART NUMBERDESCRIPTION477486 eCAP dsDNA 20,000 KitThe eCAP dsDNA 20,000 Kit is designed for the analysis of doublestrandedDNA fragments from 1,000 to 20,000 base pairs. Thischemistry utilizes linear polyacrylamide in solution to form a sievinggel. A neutral capillary provided with this kit has a stable, physicallybonded polyacrylamide coating which eliminates electroosmotic flow(EOF) and suppresses interactions of the sample with the capillary wall.This chemistry utilizes a standard Tris-Borate EDTA buffer system andallows the addition of intercalating dyes like ethidium bromide or ourproprietary EnhanCE , to improve resolution and increase sensitivity.This kit is ideal for the analysis of large PCR * products which can beanalyzed directly with no sample clean-up. The system is compatiblewith both UV and LIF detection.Kit includes: (2) 100 µm I.D. neutral capillaries (DNA),(3) vials of replaceable polyacrylamide gel, (1) test mix consistingof HindIII restriction digest of λ DNA. Contains 8 fragments from125 bp to 23,130 bp. For LIF applications, we recommend the use ofthe intercalating dye EnhanCE.Reorder ComponentsBefore and after separation of DNA fragmentsderived from the complete EcoR1 digestion of a12 Kbp plasmid vs. the undigested plasmid itself.477477 eCAP Neutral Capillary (DNA) 100 µm I.D.,65 cm total length, 12 cm from window to outlet(quantity 1)477485 dsDNA 20,000 Polyacrylamide Gel Kit(lyophilized) (quantity: package of 3)477483 eCAP Test Mix, HindIII Digest8fragments from 125 to 23,130 base pairs(quantity 500 µl)477406 dsDNA 1000 Internal Standard Orange GReference Marker (quantity 1 mL)Recommended477409 EnhanCE Dye (quantity 0.5 mL)* PCR is covered by U.S. patents owned by Hoffmann-La Roche Inc.Beckman CoulterContentsIndex33