p/ace™ mdq detectors - Beckman Coulter

CE APPLICATION KITSP ROTEINA NALYSISProteins make up one of the most analytically challenging group of compounds due to their complexity and charge heterogeneity. YetCE has been an ideal analytical tool for this class of compounds. As there is no one single methodology that can cover all proteins,Beckman Coulter has put together a series of methods development kits to help you design CE methodology for protein analysis.These kits are developed as “getting started” chemistries and include 1) an isoelectric focusing kit which offers a general screen of abroad range of isoelectric points, 2) a kit to allow the separation of proteins by size using methodology analogous to SDS-PAGE,and 3) a capillary zone electrophoresis methods development kit, utilizing a neutral capillary coating to minimize protein interactionwith the wall.PART NUMBERDESCRIPTION477490 eCAP cIEF 3-10 Screening KitIsoelectric focusing (IEF) is commonly used in both research andindustrial settings for purity assessment, identity, monitoring ofdegradation products, and microheterogeneity analysis. The eCAP cIEF3-10 Kit provides an advanced alternative to slab gel isoelectricfocusing. With the automated features of CE, loading, focusing,mobilizing and detecting proteins is simple. The ampholyte in this kitcovers a wide range from pH 3.0 to 10.0. This will provide a goodstarting range to allow for screening for the pI in a single run.Resolution of proteins with as little as 0.05 pI unit differences arepossible using the eCAP Neutral Capillary which minimizes EOF andprovides sharp, focused zones.The eCAP cIEF 3-10 Screening Kit contains (2) eCAP Neutralcoated capillaries, ampholytes (3-10), cIEF polymer solution, anolyte,catholyte, pI standards and instructions for system set up and analysis.32 Karat software allows the construction of isoelectric point plotssuch that the pIs of unknowns can be calculated automatically.Reorder ComponentsProtein standards: Ribonuclease A (pI 9.45),Carbonic Anhydrase II (pI 5.9), β-LactoglobulinA (pI 5.1), CCK Flanking Peptide (pI 2.75).477441 eCAP Neutral Capillary, 50 µm I.D.,45 cm total length, 14 cm from window to outlet(quantity 1)477488 cIEF Ampholyte, 3-10(quantity 1 mL)477497 cIEF Polymer Solution(quantity 100 mL)477487 cIEF Protein StandardsIncludes:Ribonuclease A (pI 9.45)(quantity 3.84 mg)Carbonic Anhydrase II (pI 5.9)(quantity 0.32 mg)β-Lactoglobulin A (pI 5.1)(quantity 0.64 mg)CCK Flanking Peptide (pI 2.75)(quantity 0.16 mg)A calibration curve from the standards included inthe cIEF 3-10 kit. Constructed by plotting the pIsof these proteins against their migration time.Beckman CoulterContentsIndex27