p/ace™ mdq detectors - Beckman Coulter

CE APPLICATION KITSP ROTEIN A NALYSIS ( CONTINUED)PART NUMBERDESCRIPTION477445 eCAP Neutral Capillary Methods DevelopmentKit for ProteinsThe heterogeneous nature of proteins makes it almost impossible todesign a single method that will work for all. With capillary zoneelectrophoresis, analytes are resolved due to differences in expressedcharge relative to mass. A protein’s pI, its solubility state, presence ofprosthetic groups or extent of glycosylation will all impact the choiceof the appropriate buffer for the CZE analysis. Moreover, at neutral tobasic pH, the surface of the capillary is highly charged and can act asan adsorptive surface for many proteins. The eCAP Neutral CapillaryMethods Development Kit utilizes a capillary with a neutralpolyacrylamide-bonded surface, rendering it nonreactive to mostproteins. The kit also contains a series of buffers, test compounds, andmarkers to assist you in developing a method for the analysis of yourprotein.Reorder Components477441 eCAP Neutral Capillary, 50 µm I.D.,45 cm total length, 14 cm from window to outlet(quantity 1)477442 50 mM Citrate Buffer, pH 3.0 (quantity 100 mL)477443 50 mM Citrate/MES Buffer, pH 6.0(quantity 100 mL)477444 50 mM Tricine Buffer, pH 8.0 (quantity 100 mL)477446 Histamine Reference Marker (cation)(quantity 1 mL)241524 Orange G Reference Marker (anion)(quantity 1 mL)477436 Protein Test Mix1st and 120th run of acidic proteinsusing the eCAP Neutral CapillaryMethods Development Kit.Beckman CoulterContentsIndex29