Bartonella quintana

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Method: for Bartonella endocarditis, two or more antigen areused. Antigens correspond to the suspected etiologies. Forcross adsorption, 3 samples of the patient’s serum are tested(dilution 1:200) before and after reaction with antigen A andB. Western blot is then performed against antigen A and B.Non adsorbed serum displays antibodies against antigen Aand B. Adsorbed serum do not displays antibodies againstthe antigen it has been adsorbed with. If one adsorbedantigen removes both homologous and heterologousantibodies (for example, after adsorption with antigen A,antibodies against both antigen A and B disappear, whileafter adsorption with antigen B antibodies against antigen Apersist), then we can certify there is a specific reactioninduced by antigen A.Reference1. Houpikian, P. and D. Raoult. 2003. Western immunoblottingfor Bartonella endocarditis. Clin.Diagn.Lab Immunol.10:95-10211. Anatomopathology
Cutaneous lesions of bacillary angiomatosis arecharacterized by a lobular proliferation of capillaryvessels. Endothelial cells constitute the wall of neo vessels.Bacterial clusters can be visualized after Warthin starrystain, or more specifically by direct immunofluorescenceor immunohistochemistry. Histological aspect is similar tothat observed in verruga peruana lesions, but can be easilydifferentiated from Kaposi sarcoma lesions. Histologicalexam of cardiac valves during Bartonella endocarditisretrieves larges vegetations associated with a destruction ofunderlying valvular tissue. Numerous bacteria can bevisualized in the valvular tissue after Warthin starry stain orusing immunohistochemistryReferences1. Lepidi, H., P. E. Fournier, and D. Raoult. 2000. Quantitativeanalysis of valvular lesions during Bartonella endocarditis.Am J Clin Pathol 114:880-889.2. Min, K. W., J. A. Reed, and D. F. Welch. 1994.Morphologically variable bacilli of cat scratch disease areidentified by immunocytochemical labelling with antibodies toRochalimaea henselae. Am.J.Clin.Pathol. 101:607-610.3. Reed, J. A., D. J. Brigati, S. D. Flynn, N. S. Mcnutt, K. W. Min,D. F. Welch, and L. N. Slater. 1992. Immunocytochemicalidentification Rochalimaea henselae in bacillary (epithelioid)angiomatis, parenchymal bacillary peliosis, and persistentfever with bacteriema. Am.J.Surg.Pathol. 16:650-657Picture 8 Lésion cutanée au cours de l'angiomatosebacillaire
Page 1 and 2: Bartonella quintanaUpdate: 17-10-20
Page 4 and 5: References1. Maurin M, Raoult D. Ba
Page 7 and 8: chronic bacteremia, bacteria circul
Page 9 and 10: References1. Koehler JE, Sanchez MA
Page 11: References1. Maurin, M., F. Eb, J.
Page 15 and 16: 12. CultureBacteria of the genus Ba
Page 17 and 18: nodes of patients with cat scratch
Page 19 and 20: EndocarditisGentamicin 3 mg/kg/d fo
Page 21 and 22: 16s-23 sAll Bartonella F QHVE1space
Page 23 and 24: All Bartonella groELF BbHs233. CGTG
Page 25 and 26: two serotypes of Bartonella hensela
Page 27 and 28: 12.Rolain JM, Arnoux D, Parzy D, Sa
Page 29 and 30: 31.Lepidi H, Fournier PE, Raoult D.
Page 31 and 32: 51.Maurin M, Birtles R, Raoult D. C
Page 33: • Heparinized blood• Blood cult

Bartonella quintana

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