#06-5558 Accrd Manual V7 - COLA

CRITERIA FOR QUALITY LABORATORY PERFORMANCEImmunohematologyIH 1 EAre typing sera used according to manufacturer’s directions?IH 2 RIf you perform ABO typing, are patient unknown red cells tested with known Anti-A and Anti-B reagents (forwardtyping) and are the results of this testing documented?IH 3 EIf you perform ABO typing, is the patient serum tested with known A1 and B red cells to confirm the forward type(reverse typing) and are the results of this testing documented?IH 4 EIf you perform Rh (D) testing, are unknown red cells tested with a known Anti-D reagent and are the results ofthis testing documented?IH 5 EAre ABO antisera checked with a positive control each day of use and are the results documented?IH 6 EAre Rh antisera checked with positive and negative controls each day of use and are the results documented?IH 7 EIs an autologous control used to detect false positive Rh tests and are the results documented?Check for this requirement in the Rh antisera package insert.IH 8 EAre all other antisera in use checked with positive and negative controls each day of use and are the resultsdocumented?IH 9 EAre ABO reagent red cells checked with a positive control each day of use and are the results documented?IH 10 EAre antibody screening cells checked with a positive control containing at least one known antibody each dayof use and are the results documented?IH 11 EAre anti-human globulin (AHG) reagent (Coombs serum) checked with positive and negative controls each dayof use and are the results documented?Test AHG routinely for IgG only. Anti-complement activity may be checked against complement-coated RBC’sif desired, but is not required. QC for AHG can be performed in one of the following ways:• React AHG with a pre-sensitized reagent red blood cell which may be prepared commercially or by the laboratory.• Use a known antibody which is demonstrated by the addition of AHG.• Add a pre-sensitized reagent red blood cell to all negative antiglobulin tests to indicate that antiglobulinserum present in the test was not inactivated by unbound globulins or diluted by excess residual saline;therefore, negative results reflect true absence of reactivity. (Using green antiglobulin serum does not substitutefor this control.)50. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .FOR MORE INFORMATION CONTACT COLA PHONE 800.981.9883 | FAX 410.381.8611 | ON-LINE www.cola.org