Abstracts Poster Abstracts - Dr Falk

75The usefulness of fine needle aspiration in diagnosis ofpancreatic tumorsMajewski Przemyslaw, Marciniak Ryszard, Janicka Jedynska Malgorzata,Drews Michal, Marciniak PatrycjaDepartment of Clinical Pathomorphology and Department of General, Gastroenterologicaland Endocrinological Surgery, University of Medical Sciences in Poznan, PolandThe aim of this study was evaluation of usefulness of fine needle aspiration (FNA) indiagnosis of pancreatic tumors.The studies were performed on a group of 132 patients (55 women and 77 men) withclinically diagnosed pancreatic tumor 103 cases, and 23 cases of pancreatitis, as wellas 6 patients with pancreatic cyst. Patients' age ranged from 20 to 87 years (mean56.03 year). In all cases FNA was done under ultrasound or computerized tomographycontrol. In 36 cases a biopsy was done during surgery. In 26 cases cytological diagnosiswas verified by postoperative histological studies. FNA samples were routinouslystained using H&E method. In cases with diagnosed or suspected malignancyimmunohistochemical studies of CA19.9, p53 oncoprotein using ABC/HRP method wereperformed.On basis of cytological examination of FNA samples the following diagnoses weremade: normal cytological picture in 25 cases; chronic pancreatitis in 32 cases;pancreatic cyst in 7 cases; malignant tumor in 39 cases (they included adenocarcinomain 30 patients and non-differentiated carcinoma in 8 cases, and anaplastic carcinomain 1 patient) and pus in 1 case. In addition in 14 cases suspicious neoplastic cells werefound, and in next 14 cases FNA material was non-diagnostic.CA19.9 expression was found in 27/39 (69%) cases with malignant tumor and in 7/14(50%) patients in cases with suspected cells. Overexpression of p53 was found in 18/39(46%) cases with malignant tumor and in 5/14 (36%) patients with suspected cells.Immunohistochemical studies of CA19.9 and p53 allowed for diagnosis of malignanttumor in next 8 cases, in which cytological examination revealed suspected cells. In agroup of 26 FNA (21 neoplastic lesion, 5 chronic pancreatitis) histological study verifiedone case of false positive diagnosis (fibrous chronic pancreatitis) and two false negativecases (adenocarcinoma G1).Controlled FNA is good diagnostic method of tumorous pancreatic lesions. In difficultcases immunohistochemical studies help in diagnosis of neoplastic lesions.