sequencing gap - Rice Genome Annotation Project
sequencing gap - Rice Genome Annotation Project
sequencing gap - Rice Genome Annotation Project
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pHOS Vector Features• The <strong>sequencing</strong> primer sites immediately flank the cloningsite to avoid excessive re-<strong>sequencing</strong> of vector DNA.• PCR primer sites are located immediately outside of theSequencing primer sites to allow PCR amplification fortemplate preparation.• The vector continues to have the regular, good vectorfeatures such as a gene for replication (origin of replication),antibiotic resistance and multiple cloning sites where wecan cut the circular plasmid and insert desired features.TIGRTHE INSTITUTE FOR GENOMIC RESEARCH