sequencing gap - Rice Genome Annotation Project
sequencing gap - Rice Genome Annotation Project
sequencing gap - Rice Genome Annotation Project
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TIGRTHE INSTITUTE FOR GENOMIC RESEARCHCauses for <strong>gap</strong>s• Non-random shotgun library– Toxicity of genes or promoters in E. coli– Genomic DNA difficult to clone (capsularpolysaccharides)– Unstable regions (low complexity)• Sequencing problems– Hard stops• Secondary structures• Very high or low GC content• Small unit tandem repeats– Loss of signal• Homopolymeric tracts• Very high or low GC content
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