sequencing gap - Rice Genome Annotation Project
sequencing gap - Rice Genome Annotation Project
sequencing gap - Rice Genome Annotation Project
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Filling small <strong>gap</strong>s in a chromosome• GAP < 15kb:– PCR using genomic DNA template– Sequence and close PCR product• GAP > 15kb or unable to PCR:– Select <strong>gap</strong>-spanning BAC– Construct large insert library (10+kb inserts)– Sequence to low coverage (2-3x)– Scaffold mate pairs and select subclone tiling to close <strong>gap</strong>TIGRTHE INSTITUTE FOR GENOMIC RESEARCH
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