sequencing gap - Rice Genome Annotation Project
sequencing gap - Rice Genome Annotation Project
sequencing gap - Rice Genome Annotation Project
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TIGRTHE INSTITUTE FOR GENOMIC RESEARCHDead EndsA Dead End occurs when a BAC at the end of asequence contig has no BAC-end hits or FPC BACsto extend the contig further• Design primers and PCR small unique region nearend of BAC• Hybridize to BAC filters to identify candidate BACs• End sequence and fingerprint candidate BACs toselect minimal tiling BAC• Sequence and Close new tiling BAC
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