sequencing gap - Rice Genome Annotation Project
sequencing gap - Rice Genome Annotation Project
sequencing gap - Rice Genome Annotation Project
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DNA Shearing with HydroShearHydroShear Assembly Cleaning QC1 2 3 4Nebulized DNA vs HydroShear sheared DNASize Selection GelsTIGR7 ngQuant Gels Prior to Vector Ligation18ng 20ng 25 ngTHE INSTITUTE FOR GENOMIC RESEARCHPCR Products After 4 Wash Cycles1 BAC DNA, 2 sheared BAC DNA,3 sheared water, 4 sterile waterBACs assembled and checkedfor contaminant sequencesXSCQB – 3 seqs(2- Lotus japonicus, 1- Homo sapiens)XSCQC – 0XSCQD – 0XSCQE – 0
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