sequencing gap - Rice Genome Annotation Project
sequencing gap - Rice Genome Annotation Project
sequencing gap - Rice Genome Annotation Project
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Repetitive Areas• Repetitive areas are regions of high similarity within thegenome/BAC.• Sequences in these areas may be misassembled by theAssembler.• Verification of the sequence of repetitive areas:• A. Identify potential repetitive areas, using repeatFinder and other tools.• B. Classify repeats based on length, copy number, % similarity,structure and complexity.• C. If repeats are misassembled, transpose spanning clones or obtainPCR products and sequence to verify assembly.• Misassemblies can occur in two ways:TIGRTHE INSTITUTE FOR GENOMIC RESEARCH
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