Evaluation and Utilisation of Fish Protein Isolate Products

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surimi. Sucrose and sorbitol have been reported to protect against freeze denaturation ofAlaska pollock surimi (Park et al., 1988). Sucrose is usually combined with sorbitol toreduce sweetness. According to Matsumoto and Noguchi (1992) the cryoprotectantiveeffect of sugar is enhanced by adding polyphosphates, perhaps by buffering effect ofpolyphosphates on muscle pH and/or the chelation of metal ions. Arakawa and Timasheff(1982) reported that cryoprotectants increase the surface of tension of water as well as thebinding energy, preventing withdrawal of water molecules from the protein, thusstabilizing the protein.According to Park and Lin (2005) the addition of cryoprotectants is important to ensuremaximum functionality of frozen surimi because freezing induces protein denaturation andaggregation. Sucrose and sorbitol, alone or mixed at approximately 9% w/w to dewateredfish meat, serve as the primary cryoprotectants in the manufacturing of surimi. In additionsodium tripolyphosphate and tetrasodium pyropolyphosphate at 0.2 to 0.3% are commonlyused as both a chelating agent, which makes metal ions in surimi inactive and as a pHadjusting agent.Few documents have been published regarding to frozen stability of FPI.Thawornchinsombut and Park (2006) studied frozen stability of FPI under variousconditions. They recommended using cryoptotectant to FPI to prevent freeze-inducedaggregation during frozen storage. However,the kind and amount of additives areunknown. In this work frozen stability of haddock protein isolate containing variousadditives have been investigated under different storage temperatures.1.1 Objectives:The overall objective was to investigate whether HPI, which contain chemically recoveredunfolded/refolded proteins, were affected by freezing and frozen storage. Other objectiveswere:- To determine the effect of cryoprotectants on the functional properties of HPI,- To determine the rheological behaviour of test samples using Brabender® viscograph Eduring frozen storage.39
2. MATERIAL AND METHODS2.1 Haddock protein isolateHaddock (Melanogrammus aeglefinus) proteins were extracted from cut-offs using thepH-shift method. Haddock protein isolate was obtained and transferred from MPF Icelandin Grindavik to Matis in Reykjavik in 12 polyethylene (PE) bags (each about 3 Kg.) underchilled conditions (
Page 1: University of IcelandFaculty of Sci
Page 4: TuáàÜtvàThe aims of the thesis
Page 8 and 9: 3.2 Whiteness 423.2.1 Fresh HPI 423
Page 10 and 11: _|áà Éy Y|zâÜxáChapter: 11. p
Page 12 and 13: _|áà Éy gtuÄxáChapter: 11. Pro
Page 14 and 15: V{tÑàxÜ DLiterature Review1. Int
Page 16: Fish fleshSolubilisation in alkali
Page 19 and 20: Table 1: Protein recovery from proc
Page 21 and 22: same attributes of surimi. The resu
Page 23 and 24: REFERENCESBatista I. 1999. Recovery
Page 25 and 26: V{tÑàxÜ EEffects of Salt and Cry
Page 27 and 28: 1. INTRODUCTIONA fish protein solut
Page 29 and 30: est period the material, thixotropi
Page 31 and 32: after 20 seconds of operating instr
Page 33 and 34: 1 and 3 days of storage at +2˚C we
Page 35 and 36: Table 9: a* values of CPS trails du
Page 37 and 38: Table 15: WL values (%)of frozen CP
Page 39 and 40: 3.5.2 Bohlin viscosity3.5.2.1 Fresh
Page 41 and 42: Fresh CPS with 3% saltViscosity (BU
Page 43 and 44: Fresh CPS with 20% saltViscosity (B
Page 45 and 46: Samples containing 5% salt with or
Page 47 and 48: 4. CONCLUSIONSIf the CPS is going t
Page 49 and 50: Kristbergsson K and Sigfusson H. 20
Page 51: Functional Properties and Rheologic
Page 55 and 56: 2.3.6 Colour measurementsColour of
Page 57 and 58: denaturation and oxidisation of hae
Page 59: Table 7: WL values (%) of HPI trail
Page 63 and 64: Time-dependent flow behaviour of HP
Page 65 and 66: 3.5.3.2 Haddock protein isolates co
Page 67 and 68: 3.5.3.4 Haddock protein isolates co
Page 69 and 70: REFERENCESArakawa T and Timasheff S
Page 71 and 72: Yataka Shimizu and Harohiko Toyohar
Page 73 and 74: Effects of Fish Protein Isolate on
Page 75 and 76: Table 2: Composition (%) of the thr
Page 77 and 78: putting samples into a hot-air oven
Page 79 and 80: isolate, significantly affected the
Page 81 and 82: 3.6.1 FlavourSoapy taste was detect
Page 83 and 84: Mean scoresSpicy smell5045403530Day
Page 85 and 86: 3.6.2 TextureIn fresh fish balls th
Page 87 and 88: Figure 7: Spider plot of sensory at
Page 89 and 90: REFERENCESEndres JG. 2001 . Soy Pro
Page 91 and 92: V{tÑàxÜ HOverall Conclusions and
Page 93 and 94: 2.3 Effects of storage time on fres
Page 95 and 96: 3. CONCLUSIONS AND RECOMMENDATIONSF
Page 97 and 98: Annex 1. Work planPhase 11.1 Fresh
Page 99 and 100: 2.2 Stability Assessment of Fresh a
Page 101 and 102: Annexe: 2Test samples photos2.1 Fre
Page 103 and 104:
2.6 Fish balls preparation and step
Page 105 and 106:
Annex: 4An Introduction to fish spe
Page 107 and 108:
4.2 ATLANTICCOD(Gadus morhua)Cod is
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Evaluation and Utilisation of Fish Protein Isolate Products

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