Oral Presentations - Pathology and Laboratory Medicine - University ...

Post-doctoral FellowAbstract # 53Kai Yu, Jayachandran N KizhakkedathuCentre for Blood Research, Department of Pathology and Laboratory Medicine,University of British of ColumbiaKai Yusynthesis of biomimicking glycopolymer brushescontaining different carbohydrate residues and theirnon-biofouling propertiesBackround/ObjectivesThe glycocalyx is a “carbohydrate-rich coat” on the external surface of the plasma membrane of cells, playing essentialrole in cell recognition, cell adhesion, protection and permeability barrier. Moreover, the glycocalyx also serves asa layer which prevents the undesirable nonspecific adhesion of other proteins and cells. Several approaches havebeen used to develop glycocalyx-mimetic surfaces to prevent non-specific adsorption either by physical adsorptionor covalent coupling carbohydrate containing polymers onto the surface. However, those surfaces process lowcarbohydrate density or being lack of functional carbohydrate residues. Thus, the development of surface with highcarbohydrate density and different carbohydrate species will address the role of carbohydrates in preventing nonspecificadsorption and provide a better non-biofouling surface.MethodsThree glycopolymer brushes containing different carbohydrate units were prepared on silicon substrates by surfaceinitiatedatom transfer radical polymerization (SI-ATRP). The formation of glycopolymer brushes were characterizedby ellipsometry, ATR-FTIR, water contact angle analysis and X-ray photoelectron spectroscopy. The kinetics ofpolymerization in solution was thoroughly studied by GPC and NMR. The capability of prepared surfaces againstadsorption from bovine serum albumin (BSA) and fibrinogen (Fb) solutions was investigated by using fluorescencemicroscope.ResultsThree novel N-substituted acrylamide derivatives containing different carbohydrate units (mannose, galactose andglucose) were synthesized by the protection-deprotection method. Homo-glycopolymer brushes were prepared bySI-ATRP of above monomers. The utilization of CuCl/CuCl2/Me6TREN as the catalyst, H2O as the solvent gavebetter control of brush growth compared to other systems. The kinetic study showed that the initial rate of thepolymerization in solution and growth of polymer brushes was very fast. The resulting three glycopolymer brushesshowed super-low protein adsorption from bovine serum albumin (BSA) and fibrinogen (Fb) solution.ConclusionOur results indicate that the glycopoymer brushes show good performance in preventing non-specific proteinadsorption. We anticipate that this new glycopolymer brushes may have applications in the development of novelnon-biofouling surfaces for medical implants, tissue engineering matrixes, drug delivery systems, biosensors anddiagnostic array.64 2 0 1 0 * P o s t e r P r e s e n t a t i o n s