Oral Presentations - Pathology and Laboratory Medicine - University ...

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Post-doctoral FellowAbstract # 75Annelein Stax, Lenka Allan, Dong Jun Zheng, Peter van den ElzenDepartment of Pathology, University of British Columbia, Vancouver, CanadaAnnelein Staxsulfatide-reactive t cells in multiple sclerosisBackround/ObjectivesMultiple Sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system. The majority ofmyelin consists of lipids (70 percent). These lipids are presented by antigen presenting cells (APC) via CD1 moleculesand recognized by CD1-restricted T cells. Apolipoprotein E (ApoE), a lipoprotein present in cerebrospinal fluid, hasbeen shown to promote capture and presentation of lipids.MethodsLipids were isolated from bovine brain myelin using sucrose density ultracentrifugation, chloroform:methanolextraction and purification using an aminopropyl and C18 column. Also, commercially available lipids or synthesizedsulfatide were used. Lipid presentation was studied by culturing CD1-restricted T cells with CD1-transfected K562cells in the presence or absence of ApoE.ResultsFrom all studied commercially available myelin lipids, only sulfatide showed to induce IFN-gamma productionby CD1-restricted T cells. This activity was reduced when adding anti-CD1 blocking antibodies to the culture.Treatment of sulfatide with HCl removed the sulfate group, resulting in galactocerebroside and reduced the reactivityof the lipid. Similar results were found when isolated myelin lipids from bovine brain or synthesized sulfatide wereused. The later one also demonstrated enhanced reactivity when cultured in the presence of ApoE.ConclusionSulfatide, one of the most abundant lipids in myelin, is presented to human CD1-restricted T cells and ApoE canenhance this presentation. This implies that sulfatide could be a potential autoantigen in MS. In addition, ApoE mayplay a role in mediating an immune response against myelin lipids. These findings have important implications for thepathogenesis of MS and the development of new therapeutic applications.86 2 0 1 0 * P o s t e r P r e s e n t a t i o n s
IndexAAbozina A. 45Abraham T. 55Allan L. 32, 84, 86Allard M. 29, 36, 37, 38, 39, 40, 44Al-Rawahi G. 80Al-Riyami A. 11Al-Sarraf A. 12, 51Anantha M. 42Andersen R. 30Anderson K. 53Andolfatto, G. 14Ang L. 29Aparicio S. 56Apel-Sarid L. 69BBaker M. 81Bally M. 42, 43Bashashat A. 26Baybik J. 18Bell L. 56Berean K. 53Bischoff L. 85Bleackle C. 29Boivin W. 28, 29, 55Bondy G. 44Book L. 80Bossé Y. 77Brooks D. 62Bryce E. 54Burgess B. 81Byrne A. 69CCarrier M. 56Carter I. 25Cetinbas N. 35Champagne S. 13Chari R. 65, 66, 67Chaworth-Musters T. 47Cheng J. 60, 71Cheng S. 72Chi K. 18Chin L. 77Chipperfield K. 11Chiu N. 58Choo J.R. 71Chow C. 71Chow E. 7 4Chow K. 56Chu F. 46Chung B.K. 32, 84Clarke B. 16Cochrane D. 69Constantinescu I. 63Côté H. 24, 45, 46, 47, 48DDai D. 22Dai L. 85Daley G. 20Dedhar S. 43DeValle N. 81Devlin A. 49Dunham C. 69Dunn S. 43EEinhorn W. 20Elliott W.M. 38El-Naggar A. 78Enfield K. 65FFawcett C. 75Forbes J. 47Frohlich J. 51Fung F. 18GGadawski I. 24, 48Gale N. 56Gao D. 17, 71Gao G. 62, 68Gardy J. 57Garmaroudi F. 26Garnis C. 52, 53Garratt J. 17Gazdar A. 65, 66Gelmon K. 43Gershom E. 73Gilks B. 16, 17, 56Glier M. 49Gosh S. 49Grant J. 54Granville D. 28, 29, 55Guns E. 58HHackett T. 77Hamilton J. 63Hamilton S. 74Hancock R. 62Haque M. 12Hardenberg G. 85Harris M. 24, 44Harvard C. 74, 75Hassal E. 19Hemida G. 82Hemida M. 27Hernandez-Lee. J. 50Hiebert P. 55Hirsch-Reinshagen V. 81Hoang L. 70Holden J. 74, 75Holmes D. 21Hughes S. 52Hull M. 24Huntsman D. 15, 56, 57IImam T. 47, 48Innis S. 61JJanes K. 26, 61Jewell K. 57Jia W. 58Jitratkosol M. 24, 47Johns K. 44Joseph C. 60KKaliciak H. 59Kalloger S. 56Kalra J. 43Keller B. 60, 61Kelly N. 70Kennett J. 31Kindrachuk J. 62Kizhakkedathu J. 62, 63, 64Kyluik D. 83LLam L. 66Lam S. 65, 66Lam W. 31, 65, 67Lapointe N. 47Lau S. 71Lee A. 15Lee Carol 38Lee Charles 38Leung C. 36, 37, 38, 41Leung S. 17, 71Levings M. 23, 85Lewis M. 74Lewis S. 75Lichtenstein S. 39Liu X. 75
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PathDay: Keynote Speaker (4:30 pm)T
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Conference Outline2010abstract #14
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Table of Contentabstract #57 The ro
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ResidentClinical SciencesArwa Al-Ri
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ResidentTitus Wong 1 , Marc Romney,
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ResidentD. Turbin 1 , D. Gao 2 , J.
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ResidentDavid F Schaeffer 1 , Eric
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ResidentMajid Zolein 1 , Daniel T.
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Graduate StudentAshish K. Marwaha 1
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Graduate StudentAmanda Vanden Hoek
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Graduate StudentXin Ye 1 , Mary Zha
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Graduate StudentLisa S. Ang 1 , Sar
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Graduate StudentAbstract # 22Brian
Page 36 and 37: OtherAbstract # 25Crystal Leung, Li
Page 38 and 39: OtherAbstract # 27Lise Matzke 1 , W
Page 40 and 41: Graduate StudentAbstract # 29Varun
Page 42 and 43: Graduate StudentAbstract # 31Maite
Page 44 and 45: Post-doctoral FellowAbstract # 33Ra
Page 46 and 47: Graduate StudentAbstract # 35Hayley
Page 48: Post-doctoral FellowAbstract # 37Es
Page 51 and 52: ResidentAhmad Al-Sarraf MD 1, 2 , G
Page 53 and 54: OtherRebecca Towle 1 , Danielle Mac
Page 55 and 56: Graduate StudentPaul R. Hiebert 1,2
Page 57 and 58: Graduate StudentV. Montoya 1 , J. G
Page 59 and 60: OtherWalter Martz and Henry Kalicia
Page 61 and 62: OtherKatelyn J. Janzen 1 , Elizabet
Page 63 and 64: Graduate StudentJasmine L. Hamilton
Page 65 and 66: Graduate StudentIan M. Wilson 1 , K
Page 67 and 68: Graduate StudentKelsie L. Thu 1,3 ,
Page 69 and 70: OtherLiat Apel-Sarid 1 , Doug Cochr
Page 71 and 72: Graduate StudentJennifer R. Choo 1,
Page 73 and 74: Graduate StudentEdwin S. Gershom 1
Page 75 and 76: OtherYing Qiao 1, 2 , Chansonette H
Page 77 and 78: Graduate StudentLeslie YM Chin 1,4
Page 79 and 80: Graduate StudentBillie Velapatiño
Page 81 and 82: Graduate StudentSophie Stukas 1 , S
Page 83 and 84: Graduate StudentKyluik DL and Scott
Page 85: Post-doctoral FellowJoel Montane 1
Page 89: Ye X. 27, 82Yee S. 31Yoshida E. 12Y
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