Oral Presentations - Pathology and Laboratory Medicine - University ...

Graduate StudentKelsie L. Thu 1,3 , Raj Chari 2,3 , Wan L. Lam 2,31University of British Columbia, Interdisciplinary Oncology Program, 2 University of BritishColumbia, Department of Pathology and Laboratory Medicine, 3 British Columbia CancerResearch CentreKelsie Thufrequent dna deletion of miRNA-101, a candidatetumour suppressor, in lung cancerAbstract # 56Backround/ObjectivesTumour suppressor gene (TSG) inactivation is a frequent event in lung cancer that can lead to cellular transformation.DNA deletions can result from selection against TSGs during tumourigenesis, making these genetic events markers forTSG discovery. The objective of this study was to discover miRNAs with novel tumour suppressor functions that aredisrupted by DNA deletion in non-small cell lung cancer (NSCLC).MethodsDNA copy number profiles for 43 NSCLC cell lines and 261 NSCLC tumours, generated by single nucleotidepolymorphism and comparative genomic hybridization arrays, respectively, were investigated to determine thefrequency of loss for all miRNAs annotated in RefSeq’s Hg18 human genome build. Publically available miRNA/mRNA expression profiles for 39/44 cell lines were obtained to determine which miRNAs had strong associationsbetween DNA deletion and miRNA expression, and of those miRNAs, which had expression levels negativelycorrelated with target mRNA expression.ResultsIn total, 105 miRNAs were present in frequent (>40%) regions of deletion, seven of which were strongly associatedwith gene expression (p