10th INTERNATIONAL VERTICILLIUM SYMPOSIUM 16-20 ...

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VEGETATIVE COMPATIBILITY GROUPS OF VERTICILLIUMDAHLIAE ISOLATES OBTAINED FROM CULTIVATED ANDWEED PLANTS IN CRETED.J. VAKALOUNAKIS AND E.K. LIGOXIGAKISNational Agricultural Reaserch Foundation (N.A.G.RE.R.), Plant Protection Institute GR 71003Heraklio, Crete, GreeceE-mail: vakalounakis@nagref.grA collection of one hundred and twenty two (122) isolates of Verticilliumdahliae Kleb. obtained from 23 cultivated plant species (in open fields and undercover) belonging to nine botanical families, and seven weed species belonging to fivebotanical families, grown throughout Crete, were tested by vegetative compatibilitygroup (VCG) analysis. Most of these isolates had been classified to pathotypes andraces in previous works. The aim was to study the genetic relatedness of the isolatesand to classify them in one of the four known VCGs (1, 2, 3, 4) by pairings ofcomplementary nitrate-nonutilizing (nit) mutants induced on a chlorate-containingmedium, using tester strains from the USA. Of these: one isolate originating from anolive tree cultivated at Messara valley was assigned to VCG1; 27 isolates (obtainedfrom 10 cultivated species belonging to six families and two weed species belongingto two families) were assigned to VCG2, and 62 isolates (obtained from 17 cultivatedspecies belonging to seven families and seven weed species belonging to fivefamilies) were assigned to VCG4. No isolates were assigned to VCG3. All isolateswithin a VCG were strongly compatible with at least one of the tester isolates butwere not always completely incompatible with tester strains of other VCGs. Thirteenisolates (obtained from eight cultivated species belonging to 5 families and a weedspecies) were not assigned to a VCG because their nit mutants complmented with nitmutants of more than one tester strains. Four isolates (obtained from three cultivatedand one weed species belonging to one family) were not assigned to VCG becausethey did not produce nit mutants; whereas two other isolates (obtained from twocultivated species belonging to two families) were not assigned to a VCG becausethey produced nit mutants that did not complement any of the tester strains. Of the 89V. dahliae isolates that were finally assigned to VCGs, 68.9% belonged to VCG4,30.0% to VCG2 and 1.1% to VCG1. This is the first attempt Cretan isolates of V.dahliae to be assigned to VCGs . and the second report that VCG1 is recorded in ourcountry.100
DETERMINATION OF PATHOTYPES OF VERTICILLIUMDAHLIAE AND NEW HOSTS OF V. DAHLIAE RACE-2, ANDSPREAD OF THE PATHOGEN IN CRETEE.K. LIGOXIGAKISNational Agricultural Reaserch Foundation (N.A.G.RE.R.), Plant Protection Institure GR 71003 Crete,GreeceE-mail: nagrefhe@otenet.grVerticillium dahliae Kleb. is a soil-borne fungus infecting more than 400cultivated and weed plant species, worldwide, causing usually severe disease andsubstantial damage. Two physiological races (1 and 2) and several pathotypes of thepathogen have been identified. A collection of 105 V. dahliae isolates obtained from37 plant species (25 cultivated and 12 weed) belonging to 13 botanical families andgrown on several areas of Crete was used in the present study, with the purpose toidentify pathotypes and races.Crassification of isolates to pathotypes was attempted by examining theirpathogenicity, using the root-dipping technique on four differential plant species(eggplant, pepper, tomato and turnip). Of the 105 V. dahliae isolates: 71 obtainedfrom 31 infected species (22 cultivated and 9 weed) belonging to 10 botanic families,were determined to the pathotype I (proved pathogenic to tomato, eggplant, andturnip), 26 obtained from 16 species (10 cultivated and 6 weed) belonging to 7botanical families were classified to the pathotype II (proved pathogenic to eggplantand turnip), whereas the remaining 8, obtained from 2 cultivated species belonging toone botanical family were classified to the pathotype III (proved pathogenic to all fourdifferential species). The conclusions are: a) the pathotypes I, II, and III of V. dahliaewere greatly, moderately, and lightly spread, respectively, in Crete, and b) thepathotype IV (isolates pathogenic only to turnip, according to Japanese workers),lacks in Crete.Crassification of isolates to races was attempted by examining theirpathogenicity, using two differential tomato varieties: Earlypack No 7 (lacking the Veresistance gene) and ACE 55 VF (possessing the Ve gene), using the root-dippingtechnique. Of these 105 V. dahliae isolates, 50 obtained from 21 cultivated species:bean * , broccoli * , cabbage * , cauliflower * , chickpea, chicory * , cucumber * , eggplant,endive * , melon, ochrus pea * , olive tree, pepper * , potato * , Romaine lettuce, radish * ,summer squash, masigold * , tomato, vetch * , and watermelon, as well as 17 isolatesobtained from 9 weed species: black nightshade, common groundsel * , Erodium sp. * ,field bindweed * , hoary cress * , shepherd’s purse * , senape * , Trifolium sp. * , and wildradish * proved to belong to race-2. Of the remaining 38 isolates, 12 obtaining from 10species (9 cultivated and one weed) proved to belong to race-1, whereas 26 obtainedfrom 16 species (10 cultivated and 6 weed) proved non-pathogenic to tomato. Theconclusions are the identification of: a) 20 worldwide new hosts of race-2 (thesewhich are listed with asterisks) and b) 10 known hosts of it (these which are listedwithout asterisks). Race 2 of the fungus proved widely spread in Crete infecting Vetomatoes, vegetables, olive trees, etc (such as in the Lasithi plateau and the Messaravalley areas). A 84.8% of the pathogenic to tomato isolates used belong to race-2,whereas the remaining 15.2% belong to race-1. Today because of its spread, race-2 isa serious problem to many vegetables in Crete.101
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10th INTERNATIONAL VERTICILLIUMSYMP
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10th INTERNATIONAL VERTICILLIUM SYM
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ORAL PRESENTATIONSTAXONOMY AND GENE
Page 10 and 11:
12.00 MANOLIS MARKAKISQuantificatio
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16.40 MALTE BEINHOFFDetection and f
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Production and scavenging of ROS du
Page 16 and 17:
C.Lucena 1 , J.A.J.Berni 2 , M.Mont
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15.10 MARIA DOLORES GARCÍA-PEDRAJA
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1 Agricultural Research Organizatio
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17.15 CLOSING REMARKS17.30ELECTION
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VERTICILLIUM COMPARATIVE GENOMICSST
Page 27 and 28:
ANALYSES OF MATING TYPE GENES IN VE
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DEVELOPMENT AND APPLICATION OF NEW
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QUANTITATIVE DETECTION OF MULTIPLE
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ANALYSIS OF VERTICILLIUM LONGISPORU
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CHORISMATE SYNTHASE AND COLONIZATIO
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CHANGES IN ETHYLENE PERCEPTION OF A
Page 39 and 40:
IDENTIFICATION OF THE V. DAHLIAE SE
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MOLECULAR AND GENETIC DETERMINANTS
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CHARACTERIZATION AND GENETICS OF DI
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THE SUCROSE NON FERMENTING PROTEIN
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QTL MAPPING OF VERTICILLIUM RESISTA
Page 49 and 50: QUANTIFICATION OF DEFOLIATING AND N
Page 51 and 52: CATALYTIC SUBUNIT OF PROTEIN KINASE
Page 53 and 54: IDENTIFICATION AND CHARACTERIZATION
Page 55 and 56: AUTOPHAGY AND RESTING STRUCTURE DEV
Page 57 and 58: WILTING DISEASE IN THE HALLERTAUER
Page 59 and 60: CHARACTERISATION OF POTENTIAL PATHO
Page 61 and 62: CHARACTERIZATION OF NEP-LIKE PROTEI
Page 63 and 64: INSIGHTS INTO THE ROLE OF THE NECRO
Page 65 and 66: VERTICILLIUM LONGISPORUM-INDUCED GE
Page 67 and 68: IN VITRO ELICITATION OF PATHOGENICI
Page 69 and 70: ON VERTICILLIUM DAHLIAE PLASTICITY
Page 71 and 72: PHYSIOLOGICAL DIFFERENCES EXPRESSED
Page 73 and 74: PRODUCTION AND SCAVENGING OF ROS DU
Page 75 and 76: VERTICILLIUM WILT OF OLIVES IN SOUT
Page 77 and 78: BIOLOGICAL CONTROL OF VERTICILLIUM
Page 79 and 80: THE INFLUENCE OF AGRONOMIC FACTORS
Page 81 and 82: HIGH RESOLUTION THERMAL REMOTE SENS
Page 83 and 84: STENOTROPHOMONAS - NEW INSIGHTS INT
Page 85 and 86: CONTROL OF VERTICILLIUM WILT OF OLI
Page 87 and 88: MICROBIAL ANTAGONISTS AND COMPOST-B
Page 89 and 90: ATTEMPTS TO CONTROL VERTICILLIUM WI
Page 91 and 92: VERTICILLIUM RESISTANCE IN MAPLEJEL
Page 93 and 94: INITIAL SURVEY OF VERTICILLIUM WILT
Page 95 and 96: DETECTION AND QUANTIFICATION OF VER
Page 97 and 98: VERTICILLIUM INTERSPECIFIC HYBRIDS
Page 99: AN OVERVIEW OF VEGETATIVE COMPATIBI
Page 103 and 104: POTENTIAL OF LIGNIN INCORPORATION I
Page 105 and 106: A KNOWLEDGE-BASED SYSTEM TO PREDICT
Page 107 and 108: EFFECTS OF ACIBENZOLAR-S-METHYL ON
Page 109 and 110: MOLECULAR ANALYSIS OF ENDOPHYTIC CO
Page 111 and 112: AN OUTBREAK OF VERTICILLIUM WILT IN
Page 113 and 114: EFFECT OF GUANITO ON OLIVE PLANTLET
Page 115 and 116: Grisebachstr. 8D-37077 GOETTINGEN,
Page 117 and 118: DR. EVA HÄFFNERFreie Universität
Page 119 and 120: The Pennsylvania State UniversityUN
Page 121 and 122: Department of Plant Pathology75 Ier
Page 123 and 124: FAX: +31-317-483412E-mail: Partha.s
Page 125 and 126: DR. DIMITRIOS TSITSIYIANNISAgricult
Page 127 and 128: AUTHORS’ INDEXAADAM..............
Page 129 and 130: KKAMBLE ...........................
Page 131: TRAPERO-CASAS .....................
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10th INTERNATIONAL VERTICILLIUM SYMPOSIUM 16-20 ...

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