10th INTERNATIONAL VERTICILLIUM SYMPOSIUM 16-20 ...

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VERTICILLIUM DAHLIAE ON OLIVES IN TURKEY:DISTRIBUTION, VEGETATIVE COMPATIBILITY ANDMOLECULAR CHARACTERIZATION AND VIRULENCE OFSELECTED ISOLATESDERVIS S. 1 , ERTEN L. 2 , MERCADO-BLANCO J. 3 , VALVERDE-CORREDOR A. 3 AND PÉREZ-ARTÉS E. 31 Mustafa Kemal University, Department of Plant Protection, Faculty of Agriculture, 31034 Antakya,Hatay, Turkey;2 Olive Research Institute, Turkish Ministry of Agriculture and Rural Affairs, 35100 Bornova, Izmir,Turkey;3 Instituto de Agricultura Sostenible, CSIC. Alameda del Obispo s/n. 14080 Córdoba, SpainThis study reports observations made in a comprehensive survey of theVerticillium wilt of olive in 14 provinces of Turkey over 6 years. Vegetativecompatibility groups (VCGs) and PCR-based moanalyses were used to assess thedistribution of the defoliating (D) and nondefoliating (ND) pathotypes of Verticilliumdahliae in surveyed areas. Pathogen prevalence was 35.0% of all olive orchardssurveyed and incidence of the disease was 3.1%. VCG1A was the predominant(29.3%) VCG and was able to infect major cultivars (Memecik, Ayvalik, Gemlik,Domat, Yamalak Kabasi, Kilis Yaglik, Uslu, Halhali, Manzanilla, Nizip Yaglik andMaras) grown in Turkey. The other VCGs 2A and 4B were of minor relevant (5.8%).Disease incidence for VCG1A was greater (6.9-1.1%) than the VCG2A and VCG4Bin 10 provinces (Manisa, Aydin, Kahramanmaras, Izmir, Mugla, Kilis, Denizli,Gaziantep, Mardin and Balikesir) but lower (0.2-0.5%) in three provinces (Hatay,Osmaniye and Bursa) being not found in Canakkale. Bioassays (19) revealed that acontinuum of virulence within VCG1A isolates (13) and that virulence was alsorelated to susceptibility of olive genotypes (Manzanilla, Ayvalik and Gemlik) tested.PCR-based analyses (115) were carried out to assess molecular pathotyping ofrepresentative isolates and to qualify D and ND pathotypes. Specific PCR markers forD and ND pathotypes corresponded with VCG1A and VCG2A/4B isolates,respectively. Remarkably, the V. dahliae VCG1A/D pathotype population infectingolive in Turkey was molecularly different form that one previously identified inSpain. This study is also the first record of both D and ND pathotypes from olives inthe Mediterranean and D pathotype from olives in the southeastern Anatolia regionsof Turkey30
QUANTITATIVE DETECTION OF MULTIPLE VERTICILLIUMSPECIES IN SOIL USING REAL-TIME PCRDEBODE J. 1 , VAN POUCKE K. 1 , FRANCA S.C. 2 , HÖFTE M. 2 , MAES M. 1AND HEUNGENS K. 11 Plant Sciences Unit – Crop Protection, Institute for Agricultural and Fisheries Research (ILVO),Burg. van Gansberghelaan 96 bus 2, 9820 Merelbeke, Belgium. 2 Laboratory of Phytopathology,Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure links653, 9000 Ghent, Belgium. Jane.Debode@ilvo.vlaanderen.beSoils can simultaneously contain different microsclerotia-forming Verticilliumspecies such as V. dahliae, V. longisporum, and V. tricorpus. V. dahliae infects a widerange of mainly non-cruciferous hosts, V. longisporum infects almost exclusivelycrucifers, and V. tricorpus is suspected to be a beneficial biocontrol species. As evenlow numbers of microsclerotia of V. dahliae and V. longisporum can lead to highlevels of disease, and no reliable curative measures exist to control these pathogens,accurate pre-planting assessment of their inoculum potential in soil is important.Simultaneous detection of V. tricorpus may further help in the evaluation of thedisease suppressiveness of the soil.The most commonly used technique to quantify the microsclerotia of theseclosely related Verticillium species is wet sieving of the soil, followed by plating onsemi-selective medium and microscopic analysis. A first limitation of this techniqueis that morphological differentiation between microsclerotia of different Verticilliumspecies on medium is very labor-intensive and often difficult. A second limitation isthat the standard technique is usually restricted to small soil samples (12.5 g), whichlimits the sensitivity of the assay. A third limitation is that the assay takes severalweeks to complete.In this study, a method is described based on centrifugation of the soil in 70%sucrose for scaling up the amount of soil from which the microsclerotia are extracted.In addition, real-time PCR assays are developed for specific detection andquantification of V. tricorpus and V. longisporum, using primers designed to theribosomal DNA internal transcribed spacer 2 (rDNA ITS2) and the β-tubulin gene,respectively. The newly developed sampling method is combined with a previouslydescribed real-time PCR assay for V. dahliae and the new real-time PCR assays tosimultaneously detect the three Verticillium species in soil. In addition, the method isvalidated by comparing it to the conventional method using various artificially andnaturally infected field soils. The new protocol is faster (days), more reliable, andmore sensitive. This protocol can therefore help in evaluating the risk for diseasedevelopment and contribute to durable disease control strategies.31
Page 1 and 2: 10th INTERNATIONAL VERTICILLIUMSYMP
Page 3 and 4: 10th INTERNATIONAL VERTICILLIUM SYM
Page 6: ORAL PRESENTATIONSTAXONOMY AND GENE
Page 10 and 11: 12.00 MANOLIS MARKAKISQuantificatio
Page 12 and 13: 16.40 MALTE BEINHOFFDetection and f
Page 14 and 15: Production and scavenging of ROS du
Page 16 and 17: C.Lucena 1 , J.A.J.Berni 2 , M.Mont
Page 18 and 19: 15.10 MARIA DOLORES GARCÍA-PEDRAJA
Page 20 and 21: 1 Agricultural Research Organizatio
Page 22 and 23: 17.15 CLOSING REMARKS17.30ELECTION
Page 24: VERTICILLIUM COMPARATIVE GENOMICSST
Page 27 and 28: ANALYSES OF MATING TYPE GENES IN VE
Page 29: DEVELOPMENT AND APPLICATION OF NEW
Page 33 and 34: ANALYSIS OF VERTICILLIUM LONGISPORU
Page 35 and 36: CHORISMATE SYNTHASE AND COLONIZATIO
Page 37 and 38: CHANGES IN ETHYLENE PERCEPTION OF A
Page 39 and 40: IDENTIFICATION OF THE V. DAHLIAE SE
Page 41 and 42: MOLECULAR AND GENETIC DETERMINANTS
Page 43 and 44: CHARACTERIZATION AND GENETICS OF DI
Page 45 and 46: THE SUCROSE NON FERMENTING PROTEIN
Page 47 and 48: QTL MAPPING OF VERTICILLIUM RESISTA
Page 49 and 50: QUANTIFICATION OF DEFOLIATING AND N
Page 51 and 52: CATALYTIC SUBUNIT OF PROTEIN KINASE
Page 53 and 54: IDENTIFICATION AND CHARACTERIZATION
Page 55 and 56: AUTOPHAGY AND RESTING STRUCTURE DEV
Page 57 and 58: WILTING DISEASE IN THE HALLERTAUER
Page 59 and 60: CHARACTERISATION OF POTENTIAL PATHO
Page 61 and 62: CHARACTERIZATION OF NEP-LIKE PROTEI
Page 63 and 64: INSIGHTS INTO THE ROLE OF THE NECRO
Page 65 and 66: VERTICILLIUM LONGISPORUM-INDUCED GE
Page 67 and 68: IN VITRO ELICITATION OF PATHOGENICI
Page 69 and 70: ON VERTICILLIUM DAHLIAE PLASTICITY
Page 71 and 72: PHYSIOLOGICAL DIFFERENCES EXPRESSED
Page 73 and 74: PRODUCTION AND SCAVENGING OF ROS DU
Page 75 and 76: VERTICILLIUM WILT OF OLIVES IN SOUT
Page 77 and 78: BIOLOGICAL CONTROL OF VERTICILLIUM
Page 79 and 80: THE INFLUENCE OF AGRONOMIC FACTORS
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HIGH RESOLUTION THERMAL REMOTE SENS
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STENOTROPHOMONAS - NEW INSIGHTS INT
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CONTROL OF VERTICILLIUM WILT OF OLI
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MICROBIAL ANTAGONISTS AND COMPOST-B
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ATTEMPTS TO CONTROL VERTICILLIUM WI
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VERTICILLIUM RESISTANCE IN MAPLEJEL
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INITIAL SURVEY OF VERTICILLIUM WILT
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DETECTION AND QUANTIFICATION OF VER
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VERTICILLIUM INTERSPECIFIC HYBRIDS
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AN OVERVIEW OF VEGETATIVE COMPATIBI
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DETERMINATION OF PATHOTYPES OF VERT
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POTENTIAL OF LIGNIN INCORPORATION I
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A KNOWLEDGE-BASED SYSTEM TO PREDICT
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EFFECTS OF ACIBENZOLAR-S-METHYL ON
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MOLECULAR ANALYSIS OF ENDOPHYTIC CO
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AN OUTBREAK OF VERTICILLIUM WILT IN
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EFFECT OF GUANITO ON OLIVE PLANTLET
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Grisebachstr. 8D-37077 GOETTINGEN,
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DR. EVA HÄFFNERFreie Universität
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The Pennsylvania State UniversityUN
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Department of Plant Pathology75 Ier
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FAX: +31-317-483412E-mail: Partha.s
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DR. DIMITRIOS TSITSIYIANNISAgricult
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AUTHORS’ INDEXAADAM..............
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KKAMBLE ...........................
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TRAPERO-CASAS .....................
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10th INTERNATIONAL VERTICILLIUM SYMPOSIUM 16-20 ...

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