10th INTERNATIONAL VERTICILLIUM SYMPOSIUM 16-20 ...

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IDENTIFICATION OF LETTUCE GENES DIFFERENTIALLYEXPRESSED IN A VERTICILLIUM DAHLIAE-LETTUCEINTERACTION BY SUPPRESSION SUBTRACTIVEHYBRIDIZATIONSTEVEN J. KLOSTERMAN 1 , AMY ANCHIETA 1 , KARUNAKARANMARUTHACHALAM 2 , RYAN J. HAYES 1 AND KRISHNA V. SUBBARAO 21 USDA-Agricultural Research Service, 1636 E. Alisal St, Salinas, CA, USA;2 University of California, Davis, 1636 E. Alisal St, Salinas, CA, USAThe method of suppression subtractive hybridization (SSH) was employed toidentify lettuce (Lactuca sativa) genes that are differentially expressed insymptomatic leaves during a lettuce-V. dahliae interaction. A subset of cDNAs fromthe forward-(symptomatic) and reverse-subtracted (asymptomatic) SSH libraries weresequenced and subjected to homology searches using several databases. Genesidentified as expressed only in symptomatic leaves included those with homology topathogenesis-related (PR) protein genes such as PR-5 (thaumatin-like protein), PR-3(endochitinase), PR-1 (unknown function), PR-12 (defensin), and a putative lettucecysteine protease (LsCP1). In contrast, of those clones identified as differentiallyexpressed in the asymptomatic leaf tissue, only cDNAs sharing homology with PR-14(lipid transfer protein) were identified. Increased expression of PR-3 and PR-5 insymptomatic leaves was confirmed by quantitative RTPCR, and LsCP1 was onlyexpressed in symptomatic leaves. LsCP1 shares homology with plant cysteineproteases that are expressed in senescing leaves and may contribute to the leafsymptoms observed in this lettuce-V. dahliae interaction.38
IDENTIFICATION OF THE V. DAHLIAE SECRETOME BYSEQUENCE CHARACTERISTICSPARTHASARATHY SANTHANAM, RONNIE DE JONGE AND BART P H JTHOMMALaboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen,The NetherlandsPhytopathogenic fungi deliver effector proteins into the host extracellularspace (apoplastic effectors) or into the host cell (cytoplasmic effectors) where theyinteract with their targets. Large effector repertoires have been identified frombacterial pathogens, and an intrinsic biological function has been uncovered for agrowing number of these effectors. Besides bacterial pathogens, the number ofidentified (potential) effector molecules of filamentous fungal and oomycete plantpathogens is also growing. Based on the presence of the RxLR host targeting motif, itis currently predicted that the genomes of several oomycete plant pathogens encodehundreds of effectors. Nevertheless, the intrinsic biological function of only a fewfilamentous pathogen effector molecules has been identified so far. Sequencecharacteristics of fungal effectors are that they contain an N-terminal secretiondomain, and many effectors are rich in cysteines. Identification and characterizationof these unknown effectors will provide insight into the host pathogen interactions.With the availability of the Verticillium (V. dahliae and V. albo-atrum)genome sequences we attempted to predict the secreted proteins (secretome). Thisprediction was based on the results from a number of programs that predict thepresence of an N-terminal secretion domain and the subcellular localization. Onefourth of the predicted secretome contains cell wall-degrading enzymes and one thirdare conserved hypothetical proteins. When compared with other sequenced fungalplant pathogens, the number of polysaccharide lyases is significantly higher in V.dahliae. Analysis also revealed the presence of four proteins with extracellularcysteine-rich EGF-like domain and eight necrosis-inducing proteins of the NLP-class.Proteases and protease inhibitors were predicted based on the blast resultsfrom a number of web based programs such as MEROPS, Pfam SignalP, WolfPsort,Superfamily protein database. The prediction resulted in 199 and 178 proteases in V.dahliae and V. albo-atrum, respectively. One third of these proteases are secreted.39
Page 1 and 2: 10th INTERNATIONAL VERTICILLIUMSYMP
Page 3 and 4: 10th INTERNATIONAL VERTICILLIUM SYM
Page 6: ORAL PRESENTATIONSTAXONOMY AND GENE
Page 10 and 11: 12.00 MANOLIS MARKAKISQuantificatio
Page 12 and 13: 16.40 MALTE BEINHOFFDetection and f
Page 14 and 15: Production and scavenging of ROS du
Page 16 and 17: C.Lucena 1 , J.A.J.Berni 2 , M.Mont
Page 18 and 19: 15.10 MARIA DOLORES GARCÍA-PEDRAJA
Page 20 and 21: 1 Agricultural Research Organizatio
Page 22 and 23: 17.15 CLOSING REMARKS17.30ELECTION
Page 24: VERTICILLIUM COMPARATIVE GENOMICSST
Page 27 and 28: ANALYSES OF MATING TYPE GENES IN VE
Page 29 and 30: DEVELOPMENT AND APPLICATION OF NEW
Page 31 and 32: QUANTITATIVE DETECTION OF MULTIPLE
Page 33 and 34: ANALYSIS OF VERTICILLIUM LONGISPORU
Page 35 and 36: CHORISMATE SYNTHASE AND COLONIZATIO
Page 37: CHANGES IN ETHYLENE PERCEPTION OF A
Page 41 and 42: MOLECULAR AND GENETIC DETERMINANTS
Page 43 and 44: CHARACTERIZATION AND GENETICS OF DI
Page 45 and 46: THE SUCROSE NON FERMENTING PROTEIN
Page 47 and 48: QTL MAPPING OF VERTICILLIUM RESISTA
Page 49 and 50: QUANTIFICATION OF DEFOLIATING AND N
Page 51 and 52: CATALYTIC SUBUNIT OF PROTEIN KINASE
Page 53 and 54: IDENTIFICATION AND CHARACTERIZATION
Page 55 and 56: AUTOPHAGY AND RESTING STRUCTURE DEV
Page 57 and 58: WILTING DISEASE IN THE HALLERTAUER
Page 59 and 60: CHARACTERISATION OF POTENTIAL PATHO
Page 61 and 62: CHARACTERIZATION OF NEP-LIKE PROTEI
Page 63 and 64: INSIGHTS INTO THE ROLE OF THE NECRO
Page 65 and 66: VERTICILLIUM LONGISPORUM-INDUCED GE
Page 67 and 68: IN VITRO ELICITATION OF PATHOGENICI
Page 69 and 70: ON VERTICILLIUM DAHLIAE PLASTICITY
Page 71 and 72: PHYSIOLOGICAL DIFFERENCES EXPRESSED
Page 73 and 74: PRODUCTION AND SCAVENGING OF ROS DU
Page 75 and 76: VERTICILLIUM WILT OF OLIVES IN SOUT
Page 77 and 78: BIOLOGICAL CONTROL OF VERTICILLIUM
Page 79 and 80: THE INFLUENCE OF AGRONOMIC FACTORS
Page 81 and 82: HIGH RESOLUTION THERMAL REMOTE SENS
Page 83 and 84: STENOTROPHOMONAS - NEW INSIGHTS INT
Page 85 and 86: CONTROL OF VERTICILLIUM WILT OF OLI
Page 87 and 88: MICROBIAL ANTAGONISTS AND COMPOST-B
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ATTEMPTS TO CONTROL VERTICILLIUM WI
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VERTICILLIUM RESISTANCE IN MAPLEJEL
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INITIAL SURVEY OF VERTICILLIUM WILT
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DETECTION AND QUANTIFICATION OF VER
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VERTICILLIUM INTERSPECIFIC HYBRIDS
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AN OVERVIEW OF VEGETATIVE COMPATIBI
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DETERMINATION OF PATHOTYPES OF VERT
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POTENTIAL OF LIGNIN INCORPORATION I
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A KNOWLEDGE-BASED SYSTEM TO PREDICT
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EFFECTS OF ACIBENZOLAR-S-METHYL ON
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MOLECULAR ANALYSIS OF ENDOPHYTIC CO
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AN OUTBREAK OF VERTICILLIUM WILT IN
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EFFECT OF GUANITO ON OLIVE PLANTLET
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Grisebachstr. 8D-37077 GOETTINGEN,
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DR. EVA HÄFFNERFreie Universität
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The Pennsylvania State UniversityUN
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Department of Plant Pathology75 Ier
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FAX: +31-317-483412E-mail: Partha.s
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DR. DIMITRIOS TSITSIYIANNISAgricult
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AUTHORS’ INDEXAADAM..............
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KKAMBLE ...........................
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TRAPERO-CASAS .....................
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10th INTERNATIONAL VERTICILLIUM SYMPOSIUM 16-20 ...

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