10th INTERNATIONAL VERTICILLIUM SYMPOSIUM 16-20 ...

TRANSCRIPTOME ANALYSIS ON VERTICILLIUM-INFECTEDTOMATO TO IDENTIFY GENES INVOLVED IN HOSTDEFENSEERMIS YANES-PAZ 1 , SAJID REHMAN 1 , YULING BAI 2 , RAMÓN SANTOSBERMUDEZ 3 , ORLANDO BORRÁS-HIDALGO 4 AND BART P.H.J.THOMMA 11 Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PBWageningen, The Netherlands.2 Laboratory of Plant Breeding, Wageningen University, Droevendaalsesteeg 1, 6708 PBWageningen, The Netherlands.3 Bioplants Center, Ciego de Avila, Cuba.4 Center for Genetic Engineering and Biotechnology, Havana, CubaDespite the economical importance of Verticillium spp., relatively little isknown about the molecular basis of Verticillium pathogenicity and host resistanceagainst this fungus. To identify key components involved in the interaction betweenVerticillium and tomato, comparative transcriptomics was performed on susceptibleand resistant tomato lines of the cultivar MoneyMaker inoculated with a race 1 isolateof Verticillium using cDNA-AFLP. As a resistant line, Ve1 transgenic MoneyMakerwas used (Fradin et al., 2009). A total number of 176 selective primer combinationswere tested and 1434 differentially expressed transcript-derived fragments (DE-TDFs) were identified. As the DE-TDFs that are up-regulated specifically in resistanttomato upon Verticillium inoculation (302 DE-TDFs) may be involved in hostresistance, these are functionally analyzed using virus-induced gene silencing (VIGS).The initial results of this analysis will be presented.Fradin EF, Zhang Z, Juarez Ayala JC, Castroverde CCM, Nazar RN, Robb J, Liu C-M, and ThommaBPHJ (2009). Genetic dissection of Verticillium wilt resistance mediated 1 by tomato Ve1. PlantPhysiology 150: 320-332.40